LS1 - Leica Stellaris 5 Light Sheet

 Leica SP8 - STELLARIS 5 Inverted Light Sheet Confocal Microscope

Advantages of this microscope

• Live confocal imaging with high sensitivity
• 4 internal detection channels equipped with Power HyD S
•  6 Laser (Solid State) - 405, 448, 488, 514, 561, 638nm
• White Light Laser 485-685nm Super Continuum - Tau Sense Modality
• Motorised stage - slide, dish and multiwell formats
• Z-Galvanometer Stage
• Fully Integrated Light Sheet modality
• sCMOS Camera - DFC9000 GTC DLS

Confocal Objectives

• HC PL APO 10x/0.40 CS2
• HC PL APO 20x/0.75 CS2
• HC PL APO 40x/1.30 Oil CS2
• HC PL APO 63x/1.40 OIL CS2

Light Sheet Objectives

• HC PL FLUOTAR 5x/0.15 IMM DLS
• HC APO L10x/0.30 W DLS
• HC APO L 20x/0.50 W DLS
• HC FLUOTAR L25x/0.95 W DLS

Working distances

Availible combinations of mirror cap (TwinFlect) and objectives for use in lightsheet experiments

Location

Sir Alexander Fleming building, SAF 410
SN: 8200000215

Quickstart guides

 Quickstart guide Confocal LS1 (pdf)

Tiling and Multi-position Imaging (PDF) ‌

Quickstart guide - Light Sheet LS1 Leica Stellaris Inverted (PDF)

Sample Preparation

‌Light Sheet Sample Preparation (PDF)

Using U-Shaped Glass Capillaries for Sample Mounting

LS1 Live Imaging Guide (PDF)

Leica Webinar

The Best of Both Worlds: Combining Light Sheet and Confocal Microscopy

Leica Objectives

Offline software

• Leica LAS X Lite
• FIJI