WF1 - Zeiss Axio Observer

Inverted Widefield Microscope with LED illumination Zeiss Axio Observer inverted microscope with a fully motorised stage controlled by Zen acquisition software. This system is also equipped with a large range of filter sets, LED light sources from UV to far-red, and a highly sensitive Hamamatsu Flash 4 camera for fast, low-light imaging

Advantages of this microscope

• Excellent for object tracking
• Easy to use
• Slide, dish and multiwell formats
• Sensitive, fast and long-term live experiments with little photodamage
• Multi-point / multiwell 4D imaging

Location

Sir Alexander Fleming building, SAF 409

Quickstart guide

Quickstart guide - Widefield 1 Zeiss (PDF)

WF1 Live Imaging Guide (PDF)‌

Widefield Tiling Method (wf1) (PDF)

Sample Carrier Setup (PDF)‌

Multi-position acquisition (PDF)

Stitching in ZEN Blue (PDF)

Hardware configuration

Please see the FILM wiki page - WF1 hardware configuration

Flash Camera Data (PDF)

Frame Size Calculator (XLS)

Filter and LED setup WF1 (PDF)

Zeiss Objective List (PDF)

Offline software

• ZEN Lite (requires Registration)
• FIJI