In this section
@article{Jiang:2018:10.1039/c8sc01130c,
author = {Jiang, N and Ying, G-L and Yetisen, AK and Montelongo, Y and Shen, L and Xiao, Y-X and Busscher, HJ and Yang, X-Y and Su, B-L},
doi = {10.1039/c8sc01130c},
journal = {Chemical Science},
title = {A bilayered nanoshell for durable protection of single yeast cells against multiple, simultaneous hostile stimuli},
url = {http://dx.doi.org/10.1039/c8sc01130c},
volume = {9},
year = {2018}
}
TY - JOUR
AB - Single cell surface engineering provides the most efficient, non-genetic strategy to enhance cell stability. However, it remains a huge challenge to improve cell stability in complex artificial environments. Here, a soft biohybrid interfacial layer is fabricated on individual living-cell surfaces by their exposure to a suspension of gold nanoparticles and L-cysteine to form a protecting functional layer to which porous silica layers were bound yielding pores with a diameter of 3.9 nm. The living cells within the bilayered nanoshells maintained high viability (96 ± 2%) as demonstrated by agar plating, even after five cycles of simultaneous exposure to high temperature (40 °C), lyticase and UV light. Moreover, yeast cells encapsulated in bilayered nanoshells were more recyclable than native cells due to nutrient storage in the shell.
AU - Jiang,N
AU - Ying,G-L
AU - Yetisen,AK
AU - Montelongo,Y
AU - Shen,L
AU - Xiao,Y-X
AU - Busscher,HJ
AU - Yang,X-Y
AU - Su,B-L
DO - 10.1039/c8sc01130c
PY - 2018///
SN - 2041-6520
TI - A bilayered nanoshell for durable protection of single yeast cells against multiple, simultaneous hostile stimuli
T2 - Chemical Science
UR - http://dx.doi.org/10.1039/c8sc01130c
UR - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000434243300001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR - https://pubs.rsc.org/en/content/articlelanding/2018/SC/C8SC01130C
VL - 9
ER -