BibTex format
@article{Iqbal:2014:10.1182/blood-2014-07-588046,
author = {Iqbal, MB and Johns, M and Cao, J and Liu, Y and Yu, S-C and Hyde, GD and Laffan, MA and Marchese, FP and Cho, SH and Clark, AR and Gavins, FN and Woollard, KJ and Blackshear, PJ and Mackman, N and Dean, JL and Boothby, M and Haskard, DO},
doi = {10.1182/blood-2014-07-588046},
journal = {Blood},
pages = {3646--3655},
title = {PARP-14 combines with tristetraprolin in the selective posttranscriptional control of macrophage tissue factor expression},
url = {http://dx.doi.org/10.1182/blood-2014-07-588046},
volume = {124},
year = {2014}
}
RIS format (EndNote, RefMan)
TY - JOUR
AB - Tissue factor (TF) (CD142) is a 47 kDa transmembrane cell surface glycoprotein that triggers the extrinsic coagulation cascade and links thrombosis with inflammation. Although macrophage TF expression is known to be regulated at the RNA level, very little is known about the mechanisms involved. Poly(adenosine 5′-diphosphate [ADP]-ribose)-polymerase (PARP)-14 belongs to a family of intracellular proteins that generate ADP-ribose posttranslational adducts. Functional screening of PARP-14–deficient macrophages mice revealed that PARP-14 deficiency leads to increased TF expression and functional activity in macrophages after challenge with bacterial lipopolysaccharide. This was related to an increase in TF messenger RNA (mRNA) stability. Ribonucleoprotein complex immunoprecipitation and biotinylated RNA pull-down assays demonstrated that PARP-14 forms a complex with the mRNA-destabilizing protein tristetraprolin (TTP) and a conserved adenylate-uridylate-rich element in the TF mRNA 3′ untranslated region. TF mRNA regulation by PARP-14 was selective, as tumor necrosis factor (TNF)α mRNA, which is also regulated by TTP, was not altered in PARP-14 deficient macrophages. Consistent with the in vitro data, TF expression and TF activity, but not TNFα expression, were increased in Parp14−/− mice in vivo. Our study provides a novel mechanism for the posttranscriptional regulation of TF expression, indicating that this is selectively regulated by PARP-14.
AU - Iqbal,MB
AU - Johns,M
AU - Cao,J
AU - Liu,Y
AU - Yu,S-C
AU - Hyde,GD
AU - Laffan,MA
AU - Marchese,FP
AU - Cho,SH
AU - Clark,AR
AU - Gavins,FN
AU - Woollard,KJ
AU - Blackshear,PJ
AU - Mackman,N
AU - Dean,JL
AU - Boothby,M
AU - Haskard,DO
DO - 10.1182/blood-2014-07-588046
EP - 3655
PY - 2014///
SN - 0006-4971
SP - 3646
TI - PARP-14 combines with tristetraprolin in the selective posttranscriptional control of macrophage tissue factor expression
T2 - Blood
UR - http://dx.doi.org/10.1182/blood-2014-07-588046
UR - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000347465900021&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR - https://ashpublications.org/blood/article/124/24/3646/33546/PARP-14-combines-with-tristetraprolin-in-the
VL - 124
ER -
