In this section
The Network aims to promote multi-disciplinary approaches to address challenging vaccine-related questions. This page contains a curated list of publications that highlight high-impact and collaborative approaches.
@article{Boelen:2016:10.1371/journal.pcbi.1004796,
author = {Boelen, LP and O'Neill, PK and Quigley, KJ and Reynolds, CJ and Maillere, B and Robinson, JH and Lertmemongkolchai, G and Altmann, D and Boyton, R and Asquith, R},
doi = {10.1371/journal.pcbi.1004796},
journal = {PLOS Computational Biology},
title = {BIITE: A Tool to Determine HLA Class II Epitopes from T Cell ELISpot Data},
url = {http://dx.doi.org/10.1371/journal.pcbi.1004796},
volume = {12},
year = {2016}
}
TY - JOUR
AB - Activation of CD4+ T cells requires the recognition of peptides that are presented by HLA class II molecules and can be assessed experimentally using the ELISpot assay. However, even given an individual’s HLA class II genotype, identifying which class II molecule is responsible for a positive ELISpot response to a given peptide is not trivial. The two main difficulties are the number of HLA class II molecules that can potentially be formed in a single individual (3–14) and the lack of clear peptide binding motifs for class II molecules. Here, we present a Bayesian framework to interpret ELISpot data (BIITE: Bayesian Immunogenicity Inference Tool for ELISpot); specifically BIITE identifies which HLA-II:peptide combination(s) are immunogenic based on cohort ELISpot data. We apply BIITE to two ELISpot datasets and explore the expected performance using simulations. We show this method can reach high accuracies, depending on the cohort size and the success rate of the ELISpot assay within the cohort.
AU - Boelen,LP
AU - O'Neill,PK
AU - Quigley,KJ
AU - Reynolds,CJ
AU - Maillere,B
AU - Robinson,JH
AU - Lertmemongkolchai,G
AU - Altmann,D
AU - Boyton,R
AU - Asquith,R
DO - 10.1371/journal.pcbi.1004796
PY - 2016///
SN - 1553-734X
TI - BIITE: A Tool to Determine HLA Class II Epitopes from T Cell ELISpot Data
T2 - PLOS Computational Biology
UR - http://dx.doi.org/10.1371/journal.pcbi.1004796
UR - http://hdl.handle.net/10044/1/30795
VL - 12
ER -
Clinical Senior Lecturer
Dr Christopher Chiu
vaccine.network@imperial.ac.uk
+44 (0)20 8383 2301
