In this section
@article{Gilabert:2021:10.1371/journal.ppat.1009696,
author = {Gilabert, Carbajo C and Cornell, LJ and Madbouly, Y and Lai, Z and Yates, PA and Tinti, M and Tiengwe, C},
doi = {10.1371/journal.ppat.1009696},
journal = {PLoS Pathogens},
pages = {1--34},
title = {Novel aspects of iron homeostasis in pathogenic bloodstream form Trypanosoma brucei.},
url = {http://dx.doi.org/10.1371/journal.ppat.1009696},
volume = {17},
year = {2021}
}
TY - JOUR
AB - Iron is an essential regulatory signal for virulence factors in many pathogens. Mammals and bloodstream form (BSF) Trypanosoma brucei obtain iron by receptor-mediated endocytosis of transferrin bound to receptors (TfR) but the mechanisms by which T. brucei subsequently handles iron remains enigmatic. Here, we analyse the transcriptome of T. brucei cultured in iron-rich and iron-poor conditions. We show that adaptation to iron-deprivation induces upregulation of TfR, a cohort of parasite-specific genes (ESAG3, PAGS), genes involved in glucose uptake and glycolysis (THT1 and hexokinase), endocytosis (Phosphatidic Acid Phosphatase, PAP2), and most notably a divergent RNA binding protein RBP5, indicative of a non-canonical mechanism for regulating intracellular iron levels. We show that cells depleted of TfR by RNA silencing import free iron as a compensatory survival strategy. The TfR and RBP5 iron response are reversible by genetic complementation, the response kinetics are similar, but the regulatory mechanisms are distinct. Increased TfR protein is due to increased mRNA. Increased RBP5 expression, however, occurs by a post-transcriptional feedback mechanism whereby RBP5 interacts with its own, and with PAP2 mRNAs. Further observations suggest that increased RBP5 expression in iron-deprived cells has a maximum threshold as ectopic overexpression above this threshold disrupts normal cell cycle progression resulting in an accumulation of anucleate cells and cells in G2/M phase. This phenotype is not observed with overexpression of RPB5 containing a point mutation (F61A) in its single RNA Recognition Motif. Our experiments shed new light on how T. brucei BSFs reorganise their transcriptome to deal with iron stress revealing the first iron responsive RNA binding protein that is co-regulated with TfR, is important for cell viability and iron homeostasis; two essential processes for successful proliferation.
AU - Gilabert,Carbajo C
AU - Cornell,LJ
AU - Madbouly,Y
AU - Lai,Z
AU - Yates,PA
AU - Tinti,M
AU - Tiengwe,C
DO - 10.1371/journal.ppat.1009696
EP - 34
PY - 2021///
SN - 1553-7366
SP - 1
TI - Novel aspects of iron homeostasis in pathogenic bloodstream form Trypanosoma brucei.
T2 - PLoS Pathogens
UR - http://dx.doi.org/10.1371/journal.ppat.1009696
UR - https://www.ncbi.nlm.nih.gov/pubmed/34161395
UR - https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1009696
UR - http://hdl.handle.net/10044/1/89953
VL - 17
ER -
Dr. Calvin Tiengwe
Dept. of Life Sciences,
503 Sir Ernst Chain Building,
Imperial College London,
SW7 2AZ, UK
