In this section
@article{Povelones:2013:10.1111/mmi.12089,
author = {Povelones, ML and Tiengwe, C and Gluenz, E and Gull, K and Englund, PT and Jensen, RE},
doi = {10.1111/mmi.12089},
journal = {Molecular Microbiology},
pages = {713--729},
title = {Mitochondrial shape and function in trypanosomes requires the outer membrane protein, TbLOK1},
url = {http://dx.doi.org/10.1111/mmi.12089},
volume = {87},
year = {2013}
}
TY - JOUR
AB - In an RNAi library screen for loss of kinetoplast DNA (kDNA), we identified an uncharacterized Trypanosoma brucei protein, named TbLOK1, required for maintenance of mitochondrial shape and function. We found the TbLOK1 protein located in discrete patches in the mitochondrial outer membrane. Knockdown of TbLOK1 in procyclic trypanosomes caused the highly interconnected mitochondrial structure to collapse, forming an unbranched tubule remarkably similar to the streamlined organelle seen in the bloodstream form. Following RNAi, defects in mitochondrial respiration, inner membrane potential and mitochondrial transcription were observed. At later times following TbLOK1 depletion, kDNA was lost and a more drastic alteration in mitochondrial structure was found. Our results demonstrate the close relationship between organelle structure and function in trypanosomes.
AU - Povelones,ML
AU - Tiengwe,C
AU - Gluenz,E
AU - Gull,K
AU - Englund,PT
AU - Jensen,RE
DO - 10.1111/mmi.12089
EP - 729
PY - 2013///
SN - 0950-382X
SP - 713
TI - Mitochondrial shape and function in trypanosomes requires the outer membrane protein, TbLOK1
T2 - Molecular Microbiology
UR - http://dx.doi.org/10.1111/mmi.12089
UR - http://hdl.handle.net/10044/1/59310
VL - 87
ER -
Dr. Calvin Tiengwe
Dept. of Life Sciences,
503 Sir Ernst Chain Building,
Imperial College London,
SW7 2AZ, UK
