BibTex format
@article{Tiengwe:2012:10.1016/j.celrep.2012.06.007,
author = {Tiengwe, C and Marcello, L and Farr, H and Dickens, N and Kelly, S and Swiderski, M and Vaughan, D and Gull, K and Barry, JD and Bell, SD and McCulloch, R},
doi = {10.1016/j.celrep.2012.06.007},
journal = {Cell Reports},
pages = {185--197},
title = {Genome-wide Analysis Reveals Extensive Functional Interaction between DNA Replication Initiation and Transcription in the Genome of Trypanosoma brucei},
url = {http://dx.doi.org/10.1016/j.celrep.2012.06.007},
volume = {2},
year = {2012}
}
RIS format (EndNote, RefMan)
TY - JOUR
AB - Identification of replication initiation sites, termed origins, is a crucial step in understanding genome transmission in any organism. Transcription of the Trypanosoma brucei genome is highly unusual, with each chromosome comprising a few discrete transcription units. To understand how DNA replication occurs in the context of such organization, we have performed genome-wide mapping of the binding sites of the replication initiator ORC1/CDC6 and have identified replication origins, revealing that both localize to the boundaries of the transcription units. A remarkably small number of active origins is seen, whose spacing is greater than in any other eukaryote. We show that replication and transcription in T. brucei have a profound functional overlap, as reducing ORC1/CDC6 levels leads to genome-wide increases in mRNA levels arising from the boundaries of the transcription units. In addition, ORC1/CDC6 loss causes derepression of silent Variant Surface Glycoprotein genes, which are critical for host immune evasion.
AU - Tiengwe,C
AU - Marcello,L
AU - Farr,H
AU - Dickens,N
AU - Kelly,S
AU - Swiderski,M
AU - Vaughan,D
AU - Gull,K
AU - Barry,JD
AU - Bell,SD
AU - McCulloch,R
DO - 10.1016/j.celrep.2012.06.007
EP - 197
PY - 2012///
SN - 2211-1247
SP - 185
TI - Genome-wide Analysis Reveals Extensive Functional Interaction between DNA Replication Initiation and Transcription in the Genome of Trypanosoma brucei
T2 - Cell Reports
UR - http://dx.doi.org/10.1016/j.celrep.2012.06.007
UR - http://hdl.handle.net/10044/1/59373
VL - 2
ER -
