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@article{Charlton:2015:10.1016/j.chembiol.2015.10.006,
author = {Charlton, T and Kovacs-Simon, A and Michell, S and Fairweather, N and Tate, E},
doi = {10.1016/j.chembiol.2015.10.006},
journal = {Chemistry & Biology},
title = {Quantitative lipoproteomics in Clostridium difficile reveals a role for lipoproteins in sporulation},
url = {http://dx.doi.org/10.1016/j.chembiol.2015.10.006},
volume = {22},
year = {2015}
}
TY - JOUR
AB - Bacterial lipoproteins are surface exposed, anchored to the membrane by Sdiacylglyceryl modification of the N-terminal cysteine thiol. They play important roles inmany essential cellular processes and in bacterial pathogenesis. For example,Clostridium difficile is a Gram-positive anaerobe that causes severe gastrointestinaldisease, however, its lipoproteome remains poorly characterized. Here we describe theapplication of metabolic tagging with alkyne-tagged lipid analogues, in combinationwith quantitative proteomics, to profile protein lipidation across diverse C. difficilestrains and on inactivation of specific components of the lipoprotein biogenesispathway. These studies provide the first comprehensive map of the C. difficilelipoproteome, demonstrate the existence of two active lipoprotein signal peptidasesand provide insights into lipoprotein function, implicating the lipoproteome intransmission of this pathogen.
AU - Charlton,T
AU - Kovacs-Simon,A
AU - Michell,S
AU - Fairweather,N
AU - Tate,E
DO - 10.1016/j.chembiol.2015.10.006
PY - 2015///
SN - 1074-5521
TI - Quantitative lipoproteomics in Clostridium difficile reveals a role for lipoproteins in sporulation
T2 - Chemistry & Biology
UR - http://dx.doi.org/10.1016/j.chembiol.2015.10.006
UR - http://www.cell.com/chemistry-biology/abstract/S1074-5521(15)00408-1
UR - http://hdl.handle.net/10044/1/26636
VL - 22
ER -
Prof. Ed Tate
GSK Chair in Chemical Biology
Department of Chemistry
Molecular Sciences Research Hub, White City Campus,
82 Wood Lane, London, W12 0BZ
e.tate@imperial.ac.uk
Tel: +44 (0)20 759 + ext 43752 or 45821