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@article{Broncel:2015:10.1016/j.dib.2015.06.010,
author = {Broncel, M and Serwa, RA and Ciepla, P and Krause, E and Dallman, MJ and Magee, AI and Tate, EW},
doi = {10.1016/j.dib.2015.06.010},
journal = {Data in Brief},
pages = {379--383},
title = {Myristoylation profiling in human cells and zebrafish.},
url = {http://dx.doi.org/10.1016/j.dib.2015.06.010},
volume = {4},
year = {2015}
}
TY - JOUR
AB - Human cells (HEK 293, HeLa, MCF-7) and zebrafish embryos were metabolically tagged with an alkynyl myristic acid probe, lysed with an SDS buffer and tagged proteomes ligated to multifunctional capture reagents via copper-catalyzed alkyne azide cycloaddition (CuAAC). This allowed for affinity enrichment and high-confidence identification, by delivering direct MS/MS evidence for the modification site, of 87 and 61 co-translationally myristoylated proteins in human cells and zebrafish, respectively. The data have been deposited to ProteomeXchange Consortium (Vizcaíno et al., 2014 Nat. Biotechnol., 32, 223-6) (PXD001863 and PXD001876) and are described in detail in Multifunctional reagents for quantitative proteome-wide analysis of protein modification in human cells and dynamic protein lipidation during vertebrate development by Broncel et al., Angew. Chem. Int. Ed.
AU - Broncel,M
AU - Serwa,RA
AU - Ciepla,P
AU - Krause,E
AU - Dallman,MJ
AU - Magee,AI
AU - Tate,EW
DO - 10.1016/j.dib.2015.06.010
EP - 383
PY - 2015///
SN - 2352-3409
SP - 379
TI - Myristoylation profiling in human cells and zebrafish.
T2 - Data in Brief
UR - http://dx.doi.org/10.1016/j.dib.2015.06.010
UR - http://hdl.handle.net/10044/1/25440
VL - 4
ER -
Prof. Ed Tate
GSK Chair in Chemical Biology
Department of Chemistry
Molecular Sciences Research Hub, White City Campus,
82 Wood Lane, London, W12 0BZ
e.tate@imperial.ac.uk
Tel: +44 (0)20 759 + ext 43752 or 45821