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Citation

BibTex format

@article{Wright:2013:10.1038/nchem.1830,
author = {Wright, MH and Clough, B and Rackham, MD and Kaveri, R and Brannigan, J and Grainger, M and Moss, DK and Bottrill, AR and Heal, WP and Broncel, M and Serwa, RA and Brady, D and Mann, DJ and Leatherbarrow, RJ and Tewari, R and Wilkinson, AJ and Holder, AA and Tate, EW},
doi = {10.1038/nchem.1830},
journal = {Nature Chemistry},
pages = {112--121},
title = {Validation of N-myristoyltransferase as an antimalarial drug target using an integrated chemical biology approach},
url = {http://dx.doi.org/10.1038/nchem.1830},
volume = {6},
year = {2013}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - Malaria is an infectious disease caused by parasites of the genus Plasmodium, which leads to approximately one million deaths per annum worldwide. Chemical validation of new antimalarial targets is urgently required in view of rising resistance to current drugs. One such putative target is the enzyme N-myristoyltransferase, which catalyses the attachment of the fatty acid myristate to protein substrates (N-myristoylation). Here, we report an integrated chemical biology approach to explore protein myristoylation in the major human parasite P. falciparum, combining chemical proteomic tools for identification of the myristoylated and glycosylphosphatidylinositol-anchored proteome with selective small-molecule N-myristoyltransferase inhibitors. We demonstrate that N-myristoyltransferase is an essential and chemically tractable target in malaria parasites both in vitro and in vivo, and show that selective inhibition of N-myristoylation leads to catastrophic and irreversible failure to assemble the inner membrane complex, a critical subcellular organelle in the parasite life cycle. Our studies provide the basis for the development of new antimalarials targeting N-myristoyltransferase.
AU - Wright,MH
AU - Clough,B
AU - Rackham,MD
AU - Kaveri,R
AU - Brannigan,J
AU - Grainger,M
AU - Moss,DK
AU - Bottrill,AR
AU - Heal,WP
AU - Broncel,M
AU - Serwa,RA
AU - Brady,D
AU - Mann,DJ
AU - Leatherbarrow,RJ
AU - Tewari,R
AU - Wilkinson,AJ
AU - Holder,AA
AU - Tate,EW
DO - 10.1038/nchem.1830
EP - 121
PY - 2013///
SN - 1755-4349
SP - 112
TI - Validation of N-myristoyltransferase as an antimalarial drug target using an integrated chemical biology approach
T2 - Nature Chemistry
UR - http://dx.doi.org/10.1038/nchem.1830
UR - http://hdl.handle.net/10044/1/26837
VL - 6
ER -

Contact

Prof. Ed Tate
GSK Chair in Chemical Biology
Department of Chemistry
Molecular Sciences Research Hub, White City Campus,
82 Wood Lane, London, W12 0BZ

e.tate@imperial.ac.uk
Tel: +44 (0)20 759 + ext 43752 or 45821