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Research

Biological systems - including the simplest cells - exhibit a broad range of functions to thrive in their environment. Research in the Imperial College Centre for Synthetic Biology is focused on the possibility of engineering the underlying biochemical processes to solve many of the challenges facing society, from healthcare to sustainable energy. In particular, we model, analyse, design and build biological and biochemical systems in living cells and/or in cell extracts, both exploring and enhancing the engineering potential of biology.

As part of our research we develop novel methods to accelerate the celebrated Design-Build-Test-Learn synthetic biology cycle. As such research in the Centre for Synthetic Biology highly multi- and interdisciplinary covering computational modelling and machine learning approaches; automated platform development and genetic circuit engineering ; multi-cellular and multi-organismal interactions, including gene drive and genome engineering; metabolic engineering; in vitro/cell-free synthetic biology; engineered phages and directed evolution; and biomimetics, biomaterials and biological engineering.

Publications

Citation

BibTex format

@article{Goey:2018:10.1016/j.biotechadv.2018.03.021,
author = {Goey, CH and Alhuthali, S and Kontoravdi, K},
doi = {10.1016/j.biotechadv.2018.03.021},
journal = {Biotechnology Advances},
pages = {1223--1237},
title = {Host cell protein removal from biopharmaceutical preparations: toward the implementation of quality by design},
url = {http://dx.doi.org/10.1016/j.biotechadv.2018.03.021},
volume = {36},
year = {2018}
}

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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Downstream processing of protein products of mammalian cell culture currently accounts for the largest fraction of the total production cost. A major challenge is the removal of host cell proteins, which are cell-derived impurities. Host cell proteins are potentially immunogenic and can compromise product integrity during processing and hold-up steps. There is an increasing body of evidence that the type of host cell proteins present in recombinant protein preparations is a function of cell culture conditions and handling of the harvest cell culture fluid. This, in turn, can affect the performance of downstream purification steps as certain species are difficult to remove and may require bespoke process solutions. Herein, we review recent research on the interplay between upstream process conditions, host cell protein composition and their downstream removal in antibody production processes, identifying opportunities for increasing process understanding and control. We further highlight advances in analytical and computational techniques that can enable the application of quality by design.
AU  - Goey,CH
AU  - Alhuthali,S
AU  - Kontoravdi,K
DO  - 10.1016/j.biotechadv.2018.03.021
EP  - 1237
PY  - 2018///
SN  - 0734-9750
SP  - 1223
TI  - Host cell protein removal from biopharmaceutical preparations: toward the implementation of quality by design
T2  - Biotechnology Advances
UR  - http://dx.doi.org/10.1016/j.biotechadv.2018.03.021
UR  - http://hdl.handle.net/10044/1/85952
VL  - 36
ER  -