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Research

Biological systems - including the simplest cells - exhibit a broad range of functions to thrive in their environment. Research in the Imperial College Centre for Synthetic Biology is focused on the possibility of engineering the underlying biochemical processes to solve many of the challenges facing society, from healthcare to sustainable energy. In particular, we model, analyse, design and build biological and biochemical systems in living cells and/or in cell extracts, both exploring and enhancing the engineering potential of biology.

As part of our research we develop novel methods to accelerate the celebrated Design-Build-Test-Learn synthetic biology cycle. As such research in the Centre for Synthetic Biology highly multi- and interdisciplinary covering computational modelling and machine learning approaches; automated platform development and genetic circuit engineering ; multi-cellular and multi-organismal interactions, including gene drive and genome engineering; metabolic engineering; in vitro/cell-free synthetic biology; engineered phages and directed evolution; and biomimetics, biomaterials and biological engineering.

Publications

Citation

BibTex format

@article{Sellés:2018:10.1016/j.bbapap.2017.11.005,
author = {Sellés, Vidal L and Kelly, CL and Mordaka, PM and Heap, JT},
doi = {10.1016/j.bbapap.2017.11.005},
journal = {Biochim Biophys Acta Proteins Proteom},
pages = {327--347},
title = {Review of NAD(P)H-dependent oxidoreductases: Properties, engineering and application.},
url = {http://dx.doi.org/10.1016/j.bbapap.2017.11.005},
volume = {1866},
year = {2018}
}

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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - NAD(P)H-dependent oxidoreductases catalyze the reduction or oxidation of a substrate coupled to the oxidation or reduction, respectively, of a nicotinamide adenine dinucleotide cofactor NAD(P)H or NAD(P)+. NAD(P)H-dependent oxidoreductases catalyze a large variety of reactions and play a pivotal role in many central metabolic pathways. Due to the high activity, regiospecificity and stereospecificity with which they catalyze redox reactions, they have been used as key components in a wide range of applications, including substrate utilization, the synthesis of chemicals, biodegradation and detoxification. There is great interest in tailoring NAD(P)H-dependent oxidoreductases to make them more suitable for particular applications. Here, we review the main properties and classes of NAD(P)H-dependent oxidoreductases, the types of reactions they catalyze, some of the main protein engineering techniques used to modify their properties and some interesting examples of their modification and application.
AU  - Sellés,Vidal L
AU  - Kelly,CL
AU  - Mordaka,PM
AU  - Heap,JT
DO  - 10.1016/j.bbapap.2017.11.005
EP  - 347
PY  - 2018///
SN  - 1570-9639
SP  - 327
TI  - Review of NAD(P)H-dependent oxidoreductases: Properties, engineering and application.
T2  - Biochim Biophys Acta Proteins Proteom
UR  - http://dx.doi.org/10.1016/j.bbapap.2017.11.005
UR  - https://www.ncbi.nlm.nih.gov/pubmed/29129662
UR  - http://hdl.handle.net/10044/1/53944
VL  - 1866
ER  -