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Research

Biological systems - including the simplest cells - exhibit a broad range of functions to thrive in their environment. Research in the Imperial College Centre for Synthetic Biology is focused on the possibility of engineering the underlying biochemical processes to solve many of the challenges facing society, from healthcare to sustainable energy. In particular, we model, analyse, design and build biological and biochemical systems in living cells and/or in cell extracts, both exploring and enhancing the engineering potential of biology.

As part of our research we develop novel methods to accelerate the celebrated Design-Build-Test-Learn synthetic biology cycle. As such research in the Centre for Synthetic Biology highly multi- and interdisciplinary covering computational modelling and machine learning approaches; automated platform development and genetic circuit engineering ; multi-cellular and multi-organismal interactions, including gene drive and genome engineering; metabolic engineering; in vitro/cell-free synthetic biology; engineered phages and directed evolution; and biomimetics, biomaterials and biological engineering.

Publications

Citation

BibTex format

@article{Ouldridge:2021:10.1021/acs.nanolett.0c03629,
author = {Ouldridge, T and Stan, G-B and Bae, W},
doi = {10.1021/acs.nanolett.0c03629},
journal = {Nano Letters: a journal dedicated to nanoscience and nanotechnology},
pages = {265--271},
title = {In situ generation of RNA complexes for synthetic molecular strand displacement circuits in autonomous systems},
url = {http://dx.doi.org/10.1021/acs.nanolett.0c03629},
volume = {21},
year = {2021}
}

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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Synthetic molecular circuits implementing DNA or RNA strand-displacement reactions can be used to build complex systems such as molecular computers and feedback control systems. Despite recent advances, application of nucleic acid-based circuits in vivo remains challenging due to a lack of efficient methods to produce their essential components, namely, multistranded complexes known as gates, in situ, i.e., in living cells or other autonomous systems. Here, we propose the use of naturally occurring self-cleaving ribozymes to cut a single-stranded RNA transcript into a gate complex of shorter strands, thereby opening new possibilities for the autonomous and continuous production of RNA strands in a stoichiometrically and structurally controlled way.
AU  - Ouldridge,T
AU  - Stan,G-B
AU  - Bae,W
DO  - 10.1021/acs.nanolett.0c03629
EP  - 271
PY  - 2021///
SN  - 1530-6984
SP  - 265
TI  - In situ generation of RNA complexes for synthetic molecular strand displacement circuits in autonomous systems
T2  - Nano Letters: a journal dedicated to nanoscience and nanotechnology
UR  - http://dx.doi.org/10.1021/acs.nanolett.0c03629
UR  - http://hdl.handle.net/10044/1/85880
VL  - 21
ER  -