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Research

Biological systems - including the simplest cells - exhibit a broad range of functions to thrive in their environment. Research in the Imperial College Centre for Synthetic Biology is focused on the possibility of engineering the underlying biochemical processes to solve many of the challenges facing society, from healthcare to sustainable energy. In particular, we model, analyse, design and build biological and biochemical systems in living cells and/or in cell extracts, both exploring and enhancing the engineering potential of biology.

As part of our research we develop novel methods to accelerate the celebrated Design-Build-Test-Learn synthetic biology cycle. As such research in the Centre for Synthetic Biology highly multi- and interdisciplinary covering computational modelling and machine learning approaches; automated platform development and genetic circuit engineering ; multi-cellular and multi-organismal interactions, including gene drive and genome engineering; metabolic engineering; in vitro/cell-free synthetic biology; engineered phages and directed evolution; and biomimetics, biomaterials and biological engineering.

Publications

Citation

BibTex format

@article{He:2020:10.1016/j.mec.2020.e00146,
author = {He, Q and Szczepaska, P and Yuzbashev, T and Lazar, Z and Ledesma-Amaro, R},
doi = {10.1016/j.mec.2020.e00146},
journal = {Metabolic Engineering Communications},
title = {De novo production of resveratrol from glycerol by engineering different metabolic pathways in Yarrowia lipolytica},
url = {http://dx.doi.org/10.1016/j.mec.2020.e00146},
volume = {11},
year = {2020}
}

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TY - JOUR
AB - Resveratrol is a polyphenol with multiple applications in pharma, cosmetics and food. The aim of this study was to construct Yarrowia lipolytica strains able to produce resveratrol. For this purpose, resveratrol-biosynthesis genes from bacteria and plants were expressed in this host. Since resveratrol can be produced either via tyrosine or phenylaniline, both pathways were tested, first with a single copy and then with two copies. The phenylalanine pathway resulted in slightly higher production in glucose media, although when the media was supplemented with amino acids, the best production was found in the strain with two copies of the tyrosine pathway, which reached 0.085 g/L. When glucose was replaced by glycerol, a preferred substrate for bioproduction, the best results, 0.104 g/L, were obtained in a strain combining the expression of the two synthesis pathways. Finally, the best producer strain was tested in bioreactor conditions where a production of 0.43 g/L was reached. This study suggests that Y. lipolytica is a promising host for resveratrol production from glycerol.
AU - He,Q
AU - Szczepaska,P
AU - Yuzbashev,T
AU - Lazar,Z
AU - Ledesma-Amaro,R
DO - 10.1016/j.mec.2020.e00146
PY - 2020///
SN - 2214-0301
TI - De novo production of resveratrol from glycerol by engineering different metabolic pathways in Yarrowia lipolytica
T2 - Metabolic Engineering Communications
UR - http://dx.doi.org/10.1016/j.mec.2020.e00146
UR - https://www.ncbi.nlm.nih.gov/pubmed/33014707
UR - http://hdl.handle.net/10044/1/83630
VL - 11
ER -