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Research

Biological systems - including the simplest cells - exhibit a broad range of functions to thrive in their environment. Research in the Imperial College Centre for Synthetic Biology is focused on the possibility of engineering the underlying biochemical processes to solve many of the challenges facing society, from healthcare to sustainable energy. In particular, we model, analyse, design and build biological and biochemical systems in living cells and/or in cell extracts, both exploring and enhancing the engineering potential of biology.

As part of our research we develop novel methods to accelerate the celebrated Design-Build-Test-Learn synthetic biology cycle. As such research in the Centre for Synthetic Biology highly multi- and interdisciplinary covering computational modelling and machine learning approaches; automated platform development and genetic circuit engineering ; multi-cellular and multi-organismal interactions, including gene drive and genome engineering; metabolic engineering; in vitro/cell-free synthetic biology; engineered phages and directed evolution; and biomimetics, biomaterials and biological engineering.

Publications

Citation

BibTex format

@article{McCarty:2020:10.1038/s41467-020-15053-x,
author = {McCarty, NS and Graham, AE and Studená, L and Ledesma-Amaro, R},
doi = {10.1038/s41467-020-15053-x},
journal = {Nature Communications},
title = {Multiplexed CRISPR technologies for gene editing and transcriptional regulation},
url = {http://dx.doi.org/10.1038/s41467-020-15053-x},
volume = {11},
year = {2020}
}

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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Multiplexed CRISPR technologies, in which numerous gRNAs or Cas enzymes are expressed at once, have facilitated powerful biological engineering applications, vastly enhancing the scope and efficiencies of genetic editing and transcriptional regulation. In this review, we discuss multiplexed CRISPR technologies and describe methods for the assembly, expression and processing of synthetic guide RNA arrays in vivo. Applications that benefit from multiplexed CRISPR technologies, including cellular recorders, genetic circuits, biosensors, combinatorial genetic perturbations, large-scale genome engineering and the rewiring of metabolic pathways, are highlighted. We also offer a glimpse of emerging challenges and emphasize experimental considerations for future studies.
AU  - McCarty,NS
AU  - Graham,AE
AU  - Studená,L
AU  - Ledesma-Amaro,R
DO  - 10.1038/s41467-020-15053-x
PY  - 2020///
SN  - 2041-1723
TI  - Multiplexed CRISPR technologies for gene editing and transcriptional regulation
T2  - Nature Communications
UR  - http://dx.doi.org/10.1038/s41467-020-15053-x
UR  - https://www.ncbi.nlm.nih.gov/pubmed/32152313
UR  - http://hdl.handle.net/10044/1/78004
VL  - 11
ER  -