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Research

Biological systems - including the simplest cells - exhibit a broad range of functions to thrive in their environment. Research in the Imperial College Centre for Synthetic Biology is focused on the possibility of engineering the underlying biochemical processes to solve many of the challenges facing society, from healthcare to sustainable energy. In particular, we model, analyse, design and build biological and biochemical systems in living cells and/or in cell extracts, both exploring and enhancing the engineering potential of biology.

As part of our research we develop novel methods to accelerate the celebrated Design-Build-Test-Learn synthetic biology cycle. As such research in the Centre for Synthetic Biology highly multi- and interdisciplinary covering computational modelling and machine learning approaches; automated platform development and genetic circuit engineering ; multi-cellular and multi-organismal interactions, including gene drive and genome engineering; metabolic engineering; in vitro/cell-free synthetic biology; engineered phages and directed evolution; and biomimetics, biomaterials and biological engineering.

Publications

Citation

BibTex format

@article{Ledesma-Amaro:2018:10.1021/acssynbio.8b00287,
author = {Ledesma-Amaro, R and Jiménez, A and Revuelta, JL},
doi = {10.1021/acssynbio.8b00287},
journal = {ACS Synthetic Biology},
pages = {2340--2347},
title = {Pathway Grafting for Polyunsaturated Fatty Acids Production in Ashbya gossypii through Golden Gate Rapid Assembly.},
url = {http://dx.doi.org/10.1021/acssynbio.8b00287},
volume = {7},
year = {2018}
}

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RIS format (EndNote, RefMan)

TY - JOUR
AB - Here we present a Golden Gate assembly system adapted for the rapid genomic engineering of the industrial fungus Ashbya gossypii. This biocatalyst is an excellent biotechnological chassis for synthetic biology applications and is currently used for the industrial production of riboflavin. Other bioprocesses such as the production of folic acid, nucleosides, amino acids and biolipids have been recently reported in A. gossypii. In this work, an efficient assembly system for the expression of heterologous complex pathways has been designed. The expression platform comprises interchangeable DNA modules, which provides flexibility for the use of different loci for integration, selection markers and regulatory sequences. The functionality of the system has been applied to engineer strains able to synthesize polyunsaturated fatty acids (up to 35% of total fatty acids). The production of the industrially relevant arachidonic, eicosapentanoic and docosahexanoic acids remarks the potential of A. gossypii to produce these functional lipids.
AU - Ledesma-Amaro,R
AU - Jiménez,A
AU - Revuelta,JL
DO - 10.1021/acssynbio.8b00287
EP - 2347
PY - 2018///
SN - 2161-5063
SP - 2340
TI - Pathway Grafting for Polyunsaturated Fatty Acids Production in Ashbya gossypii through Golden Gate Rapid Assembly.
T2 - ACS Synthetic Biology
UR - http://dx.doi.org/10.1021/acssynbio.8b00287
UR - https://www.ncbi.nlm.nih.gov/pubmed/30261136
UR - http://hdl.handle.net/10044/1/63485
VL - 7
ER -