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@article{Sparks:2020:10.1002/jbio.201960239,
author = {Sparks, H and Dvinskikh, L and Firth, J and Francis, A and Harding, S and Paterson, C and MacLeod, K and Dunsby, C},
doi = {10.1002/jbio.201960239},
journal = {Journal of Biophotonics},
title = {Development a flexible light-sheet fluorescence microscope for high-speed 3D imaging of calcium dynamics and 3D imaging of cellular microstructure},
url = {http://dx.doi.org/10.1002/jbio.201960239},
volume = {13},
year = {2020}
}
TY - JOUR
AB - We report a flexible lightsheet fluorescence microscope (LSFM) designed for studying dynamic events in cardiac tissue at high speed in 3D and the correlation of these events to cell microstructure. The system employs two illuminationdetection modes: the first uses angledithering of a Gaussian light sheet combined with remote refocusing of the detection plane for videorate volumetric imaging; the second combines digitallyscanned lightsheet illumination with an axiallyswept lightsheet waist and stagescanned acquisition for improved axial resolution compared to the first mode. We present a characterisation of the spatial resolution of the system in both modes. The first illuminationdetection mode achieves dual spectralchannel imaging at 25 volumes per second with 1024 × 200 × 50 voxel volumes and is demonstrated by timelapse imaging of calcium dynamics in a live cardiomyocyte. The second illuminationdetection mode is demonstrated through the acquisition of a higher spatial resolution structural map of the ttubule network in a fixed cardiomyocyte cell.
AU - Sparks,H
AU - Dvinskikh,L
AU - Firth,J
AU - Francis,A
AU - Harding,S
AU - Paterson,C
AU - MacLeod,K
AU - Dunsby,C
DO - 10.1002/jbio.201960239
PY - 2020///
SN - 1864-063X
TI - Development a flexible light-sheet fluorescence microscope for high-speed 3D imaging of calcium dynamics and 3D imaging of cellular microstructure
T2 - Journal of Biophotonics
UR - http://dx.doi.org/10.1002/jbio.201960239
UR - http://hdl.handle.net/10044/1/78056
VL - 13
ER -