BibTex format
@article{Bharadwaj:2024:1361-6501/ad761b,
author = {Bharadwaj, A and Kumar, A and Kalita, R and MITRA, R and Sharma, A and Jaganathan, BG and Bhattacharyya, J and Sarma, A and Garcia, E and Kumar, S and Lightley, J and Alexandrov, Y and Dunsby, C and Neil, M and French, PMW and Boruah, BR},
doi = {1361-6501/ad761b},
journal = {Measurement Science and Technology},
title = {Super-resolved fluorescence imaging utilising accessible stochastic optical reconstruction microscopy (easySTORM) implemented on a low-cost, modular open-source (openFrame) microscope},
url = {http://dx.doi.org/10.1088/1361-6501/ad761b},
volume = {35},
year = {2024}
}
RIS format (EndNote, RefMan)
TY - JOUR
AB - Optical super-resolution microscope is a powerful tool for the life sciences, including cell biology and pathology yielding information on, e.g. biological structure and protein distribution in the nano-scale range. In recent years, there has been an exponential rise in the interest to apply super-resolution microscopes in diverse areas, but its wider utility is hindered by the cost of purchasing and maintaining super-resolved microscopes. In this paper we present the implementation of easySTORM, an accessible implementation of stochastic optical reconstruction microscopy (STORM) at Indian Institute of Technology Guwahati (IITG), India, in a flexible, user friendly and cost-effective manner using a state-of-the-art, modular, open-source, optical microscope platform called: 'openFrame'. Providing comparable imaging performance to commercial optical super-resolution microscopes, the openFrame-based implementation of easySTORM uses in-expensive multimode diode lasers and industry grade CMOS cameras, and the open-source and modular nature of the instrument makes it easy to maintain and to upgrade. To demonstrate its successful implementation at IITG, we image quantum dots and actin-tubulin structure in both normal and cancer cells, resolved features separated by a few tens of nanometers. This work demonstrates that openFrame-enabled easySTORM instrumentation can be widely accessible to provide affordable, research grade super-resolution microscopy capability for academic and medical research.
AU - Bharadwaj,A
AU - Kumar,A
AU - Kalita,R
AU - MITRA,R
AU - Sharma,A
AU - Jaganathan,BG
AU - Bhattacharyya,J
AU - Sarma,A
AU - Garcia,E
AU - Kumar,S
AU - Lightley,J
AU - Alexandrov,Y
AU - Dunsby,C
AU - Neil,M
AU - French,PMW
AU - Boruah,BR
DO - 1361-6501/ad761b
PY - 2024///
SN - 0957-0233
TI - Super-resolved fluorescence imaging utilising accessible stochastic optical reconstruction microscopy (easySTORM) implemented on a low-cost, modular open-source (openFrame) microscope
T2 - Measurement Science and Technology
UR - http://dx.doi.org/10.1088/1361-6501/ad761b
UR - http://hdl.handle.net/10044/1/114959
VL - 35
ER -