easySTORM
easySTORM is a technique to implement single molecule localisation microscopy (SMLM) on almost any fluorescence microscope, including instruments based on our openFrame. A typical implementation comprises multimode diode lasers for excitation, multimode optical fibre (MMF) and a fibre-vibrator to provide uniform illumination with time-averaged speckle, and a set of optomechanical components for the openExcite illuminator to couple the excitation light from the MMF to the microscope (e.g. via the lamp illumination port on a commercial microscope).
For a list of components to implement easySTORM using the openFrame, please click here to download the easySTORM components. Note that you can use many of these components to upgrade an existing (commercial) fluorescnce microscope to easySTORM, as described in our J Biophotonics paper.
The figure below shows some easySTORM super-resolved images of fixed Hela cells with tubulin immunolabelled with ifluor-647 imaged using a low-cost, uncooled CMOS camera and a 1.4 NA objective lens with an openFrame microscope.
