BibTex format
@article{Stronach:2015:10.18632/oncotarget.3415,
author = {Stronach, EA and Cunnea, P and Turner, C and Guney, T and Aiyappa, R and Jeyapalan, S and de, Sousa CH and Browne, A and Magdy, N and Studd, JB and Sriraksa, R and Gabra, H and El-Bahrawy, M},
doi = {10.18632/oncotarget.3415},
journal = {Oncotarget},
pages = {31593--31603},
title = {The role of interleukin-8 (IL-8) and IL-8 receptors in platinum response in high grade serous ovarian carcinoma},
url = {http://dx.doi.org/10.18632/oncotarget.3415},
volume = {6},
year = {2015}
}
RIS format (EndNote, RefMan)
TY - JOUR
AB - Platinum based drugs are the cornerstone of chemotherapy for ovarian cancer, however the development of chemoresistance hinders its success. IL-8 is involved in regulating several pro-survival pathways in cancer. We studied the expression of IL-8 and IL-8 receptors in platinum sensitive and resistant cell lines. Using qRT-PCR and immunohistochemistry, both platinum sensitive (PEA1, PEO14) and resistant (PEA2, PEO23) show increased expression of IL-8 and IL-8 receptors. IL-8RA shows nuclear and cytoplasmic expression, whilst IL-8RB is present solely in the cytoplasm. Knockdown of IL-8 increased sensitivity to cisplatin in platinum sensitive and reversed platinum resistance in resistant cell lines, decreased the expression of anti-apoptotic Bcl-2 and decreased inhibitory phosphorylation of pro-apoptotic Bad. IL-8 receptor antagonist treatment also enhanced platinum sensitivity. Nuclear localisation of IL-8RA was only detected in platinum resistant tumours. Inhibition of IL-8 signalling can enhance response in platinum sensitive and resistant disease. Nuclear IL-8RA may have potential as a biomarker of resistant disease.
AU - Stronach,EA
AU - Cunnea,P
AU - Turner,C
AU - Guney,T
AU - Aiyappa,R
AU - Jeyapalan,S
AU - de,Sousa CH
AU - Browne,A
AU - Magdy,N
AU - Studd,JB
AU - Sriraksa,R
AU - Gabra,H
AU - El-Bahrawy,M
DO - 10.18632/oncotarget.3415
EP - 31603
PY - 2015///
SN - 1949-2553
SP - 31593
TI - The role of interleukin-8 (IL-8) and IL-8 receptors in platinum response in high grade serous ovarian carcinoma
T2 - Oncotarget
UR - http://dx.doi.org/10.18632/oncotarget.3415
UR - http://hdl.handle.net/10044/1/31262
VL - 6
ER -
