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BibTex format

@article{Peghaire:2019:10.1038/s41467-019-12897-w,
author = {Peghaire, C and Dufton, N and Lang, M and Salles, I and Ahnstroem, J and Kalna, V and Raimondi, C and Pericleous, C and Inuabasi, L and Kiseleva, R and Muzykantov, V and Mason, J and Birdsey, G and Randi, A},
doi = {10.1038/s41467-019-12897-w},
journal = {Nature Communications},
pages = {1--17},
title = {The transcription factor ERG regulates a low shear stress-induced anti-thrombotic pathway in the microvasculature},
url = {http://dx.doi.org/10.1038/s41467-019-12897-w},
volume = {10},
year = {2019}
}
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TY  - JOUR
AB  - Endothelial  cells  actively  maintain  an  anti-thrombotic  environment;  loss  of  this  protective  function may lead to thrombosis and systemic coagulopathy. The transcription factor ERG is  essential to maintain endothelial homeostasis. Here we show that inducible endothelial ERG deletion  (ErgiEC-KO)  in  mice  is  associated  with  spontaneous  thrombosis,  hemorrhages  and   systemic   coagulopathy.   We   find   that   ERG   drives   transcription   of   the   anti-coagulant   thrombomodulin  (TM),  as  shown  by  reporter  assays  and  chromatin  immunoprecipitation.  TM expression is regulated by shear stress (SS) via Krüppel-like factor 2 (KLF2). In vitro, ERG  regulates  TM  expression  under  low  SS  conditions,  by  facilitating  KLF2  binding  to  the  TM   promoter. However, ERG is dispensable for TM expression in high SS conditions. In ErgiEC-KO mice, TM expression is decreased in liver and lung microvasculature exposed to low SS but  not  in  blood  vessels  exposed  to  high  SS.  Our  study  identifies  an  endogenous,  vascular  bed- specific anti-coagulant pathway in microvasculature exposed to low SS.
AU  - Peghaire,C
AU  - Dufton,N
AU  - Lang,M
AU  - Salles,I
AU  - Ahnstroem,J
AU  - Kalna,V
AU  - Raimondi,C
AU  - Pericleous,C
AU  - Inuabasi,L
AU  - Kiseleva,R
AU  - Muzykantov,V
AU  - Mason,J
AU  - Birdsey,G
AU  - Randi,A
DO  - 10.1038/s41467-019-12897-w
EP  - 17
PY  - 2019///
SN  - 2041-1723
SP  - 1
TI  - The transcription factor ERG regulates a low shear stress-induced anti-thrombotic pathway in the microvasculature
T2  - Nature Communications
UR  - http://dx.doi.org/10.1038/s41467-019-12897-w
UR  - https://www.nature.com/articles/s41467-019-12897-w
UR  - http://hdl.handle.net/10044/1/74316
VL  - 10
ER  -
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