In this section
@article{Sezgin:2015:10.1002/cphc.201402794,
author = {Sezgin, E and Waithe, D and Bernardino, de la Serna J and Eggeling, C},
doi = {10.1002/cphc.201402794},
journal = {ChemPhysChem},
pages = {1387--1394},
title = {Spectral imaging to measure heterogeneity in membrane lipid packing},
url = {http://dx.doi.org/10.1002/cphc.201402794},
volume = {16},
year = {2015}
}
TY - JOUR
AB - Physicochemical properties of the plasma membrane have been shown to play an important role in cellular functionality. Among those properties, the molecular order of the lipids, or the lipid packing, is of high importance. Changes in lipid packing are believed to compartmentalize cellular signaling by initiating coalescence and conformational changes of proteins. A common way to infer membrane lipid packing is by using membraneembedded polaritysensitive dyes, whose emission spectrum is dependent on the molecular order of the immediate membrane environment. Here, we report on an improved determination of such spectral shifts in the emission spectrum of the polaritysensitive dyes. This improvement is based on the use of spectral imaging on a scanning confocal fluorescence microscope in combination with an improved analysis, which considers the whole emission spectrum instead of just single wavelength ranges. Using this approach and the polaritysensitive dyes CLaurdan or Di4ANEPPDHQ, we were able to image—with high accuracy—minute differences in the lipid packing of model and cellular membranes.
AU - Sezgin,E
AU - Waithe,D
AU - Bernardino,de la Serna J
AU - Eggeling,C
DO - 10.1002/cphc.201402794
EP - 1394
PY - 2015///
SN - 1439-7641
SP - 1387
TI - Spectral imaging to measure heterogeneity in membrane lipid packing
T2 - ChemPhysChem
UR - http://dx.doi.org/10.1002/cphc.201402794
UR - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000354367500012&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR - http://hdl.handle.net/10044/1/69270
VL - 16
ER -