In this section
@article{Quicke:2020:10.1117/1.NPh.7.3.035006,
author = {Quicke, P and Howe, CL and Song, P and Jadan, HV and Song, C and Knöpfel, T and Neil, M and Dragotti, PL and Schultz, SR and Foust, AJ},
doi = {10.1117/1.NPh.7.3.035006},
journal = {Neurophotonics},
title = {Subcellular resolution three-dimensional light-field imaging with genetically encoded voltage indicators},
url = {http://dx.doi.org/10.1117/1.NPh.7.3.035006},
volume = {7},
year = {2020}
}
TY - JOUR
AB - Significance: Light-field microscopy (LFM) enables high signal-to-noise ratio (SNR) and light efficient volume imaging at fast frame rates. Voltage imaging with genetically encoded voltage indicators (GEVIs) stands to particularly benefit from LFM's volumetric imaging capability due to high required sampling rates and limited probe brightness and functional sensitivity. Aim: We demonstrate subcellular resolution GEVI light-field imaging in acute mouse brain slices resolving dendritic voltage signals in three spatial dimensions. Approach: We imaged action potential-induced fluorescence transients in mouse brain slices sparsely expressing the GEVI VSFP-Butterfly 1.2 in wide-field microscopy (WFM) and LFM modes. We compared functional signal SNR and localization between different LFM reconstruction approaches and between LFM and WFM. Results: LFM enabled three-dimensional (3-D) localization of action potential-induced fluorescence transients in neuronal somata and dendrites. Nonregularized deconvolution decreased SNR with increased iteration number compared to synthetic refocusing but increased axial and lateral signal localization. SNR was unaffected for LFM compared to WFM. Conclusions: LFM enables 3-D localization of fluorescence transients, therefore eliminating the need for structures to lie in a single focal plane. These results demonstrate LFM's potential for studying dendritic integration and action potential propagation in three spatial dimensions.
AU - Quicke,P
AU - Howe,CL
AU - Song,P
AU - Jadan,HV
AU - Song,C
AU - Knöpfel,T
AU - Neil,M
AU - Dragotti,PL
AU - Schultz,SR
AU - Foust,AJ
DO - 10.1117/1.NPh.7.3.035006
PY - 2020///
SN - 2329-4248
TI - Subcellular resolution three-dimensional light-field imaging with genetically encoded voltage indicators
T2 - Neurophotonics
UR - http://dx.doi.org/10.1117/1.NPh.7.3.035006
UR - https://www.ncbi.nlm.nih.gov/pubmed/32904628
UR - http://hdl.handle.net/10044/1/82645
VL - 7
ER -
Centre Manager
Kate Hobson
Email: k.hobson@imperial.ac.uk
