NanoFCM NanoAnalyzer

The NanoFCM NanoAnalyzer is offered for CL2 work (not for airborne pathogens) Please refer to the Biosafety page (link at the bottom of this page) for more information on the documentation needed to use this instrument.

The NanoFCM NanoAnalyzer is a 2-colour analyser with two lasers (Blue 488nm and Red 640nm) that measures biological and synthetic nanoparticles, such as extracellular vesicles, nanoparticle carriers, virus, mitochondria and gold nanoparticles.

Unlike conventional flow cytometers, the NanoFCM has knobs for channel adjustments of signal intensity and events that ensures a daily alignment and optimisation of the system. Its light scattering allows for detection limits of 7nm to 1um, but works best at 10-100nm ranges for gold nanoparticles and 40-200nm ranges for biological particles. It is also capable of providing sample concentration measurements.

This cytometer is equipped with a capillary that is capable of acquiring from 0.2mL tubes. Please note that the data acquisition rate is not displayed and needs to be adjusted during the experiment. We recommend a minimum sample volume of 10μL and a stock of 0.2μL-filtered PBS for diluting.

Download a complete configuration of NanoFCM Lasers and Filters, including fluorochromes and bandpass filters. You can also find it below.

Location

Sir Alexander Fleming Building, 5th floor, Room 522.

The NanoFCM is available to any trained user 24 hours a day (subject to building access and regulations).