The Fortessa III is an 18 colour analyser with five lasers (UV 355nm, Violet 405nm, Blue 488nm, Yellow-Green 561nm and Red 640nm), a fixed optical alignment and a pressure-driven fluidics system. The flow cell is a gel-coupled cuvette as opposed to the jet-in-air system, and this combined with trigon and octagon detection systems provide increased sensitivity and resolution. This arrangement allows filter and mirror changes within the optical array to be made easily and requires no additional alignment for maximum signal strength.

It is equipped with an HTS (High Throughput System) or plate reader that is capable of reading from 96 or 384 well plates, as well as from tubes.

Forward and side scatter properties (FSC and SSC) allow for a minimum detectable particle size of 0.5 μm. The maximum data acquisition rate is 40,000 events/second. We recommend a minimum sample volume of 300 μL in standard mode and 200 μL in HTS mode

For more information, please read the BD LSRFortessa Cell Analyzer Technical Specifications and BD HTS Option.

Download a complete configuration of Fortessa III Lasers and Filters, including fluorochromes and bandpass filters. You can also find it below.

UV 355nm
Bandpass filter Mirror Fluorochrome
 379/28  410LP  BUV395
 450/50  -  Zombie UV
 530/30  505LP  BUV496
 740/35  -   BUV737
Summary of the table's contents
Violet 405nm
Bandpass filter Mirror Fluorochrome
 450/50  -   DAPI, Pacific Blue, BV421, Marina Blue, AF405 
 525/50  505LP   AmCyan, BV510, Qdot 525 
 610/20  595LP  BV605, Qdot 605
 660/20  630LP  BV650
 710/50  690LP  BV711
 780/60  750LP  BV785
Summary of the table's contents
Blue 488nm
Bandpass filter
Mirror Fluorochrome
 488/10  -   -
 530/30  505LP   FITC, AF488, GFP
 710/50  635LP  PerCP-Cy5.5, Per-CP
Summary of the table's contents
Yellow-Green 561nm
Bandpass filter Mirror Fluorochrome
 585/15  -  PE, AF546
 610/20  600LP  PE-TexasRe, PE-CF594, mCherry
 670/30  635LP  PE-Cy5
 780/60  750LP  PE-Cy7
Summary of the table's contents
Red 640nm
Bandpass filter Mirror Fluorochrome
 670/14  -  APC, AF647
 730/45  690LP  AF700
 780/60  750LP  APC-Cy7, APC-H7
Summary of the table's contents

Location

Sir Alexander Fleming Building, 5th floor, Room 532.

The Fortessa III is available to any trained user 24 hours a day (subject to building access and regulations).

General enquiries


Flow Cytometry Facility
South Kensington
Sir Alexander Fleming Building
Fifth Floor, Room 532
South Kensington Campus
Imperial College London
Exhibition Road
London SW7 2AZ, UK

flow@imperial.ac.uk
+44 (0)20 7594 5423