BibTex format
@article{Ja'afar:2015:10.1016/j.ultrasmedbio.2015.07.001,
author = {Ja'afar, F and Leow, CH and Garbin, V and Sennoga, CA and Tang, M-X and Seddon, JM},
doi = {10.1016/j.ultrasmedbio.2015.07.001},
journal = {Ultrasound in Medicine and Biology},
pages = {2990--3000},
title = {Surface Charge Measurement of SonoVue, Definity and Optison: A Comparison of Laser Doppler Electrophoresis and Micro-Electrophoresis},
url = {http://dx.doi.org/10.1016/j.ultrasmedbio.2015.07.001},
volume = {41},
year = {2015}
}
RIS format (EndNote, RefMan)
TY - JOUR
AB - Microbubble (MB) contrast-enhanced ultrasonography is a promising tool for targeted molecular imaging. It is important to determine the MB surface charge accurately as it affects the MB interactions with cell membranes. In this article, we report the surface charge measurement of SonoVue, Definity and Optison. We compare the performance of the widely used laser Doppler electrophoresis with an in-house micro-electrophoresis system. By optically tracking MB electrophoretic velocity in a microchannel, we determined the zeta potentials of MB samples. Using micro-electrophoresis, we obtained zeta potential values for SonoVue, Definity and Optison of −28.3, −4.2 and −9.5 mV, with relative standard deviations of 5%, 48% and 8%, respectively. In comparison, laser Doppler electrophoresis gave −8.7, +0.7 and +15.8 mV with relative standard deviations of 330%, 29,000% and 130%, respectively. We found that the reliability of laser Doppler electrophoresis is compromised by MB buoyancy. Micro-electrophoresis determined zeta potential values with a 10-fold improvement in relative standard deviation.
AU - Ja'afar,F
AU - Leow,CH
AU - Garbin,V
AU - Sennoga,CA
AU - Tang,M-X
AU - Seddon,JM
DO - 10.1016/j.ultrasmedbio.2015.07.001
EP - 3000
PY - 2015///
SN - 0301-5629
SP - 2990
TI - Surface Charge Measurement of SonoVue, Definity and Optison: A Comparison of Laser Doppler Electrophoresis and Micro-Electrophoresis
T2 - Ultrasound in Medicine and Biology
UR - http://dx.doi.org/10.1016/j.ultrasmedbio.2015.07.001
UR - http://hdl.handle.net/10044/1/39544
VL - 41
ER -