In this section
@article{Salehi-Reyhani:2013:10.1039/C3LC41388H,
author = {Salehi-Reyhani, A and Sharma, S and Burgin, E and Barclay, M and Cass, AEG and Neil, MEE and Ces, O and Willison, KR and Klug, D},
doi = {10.1039/C3LC41388H},
journal = {Lab on A Chip},
pages = {2066--2074},
title = {Scaling Advantages and Constraints in Miniaturized Capture Assays for Single Cell Protein Analysis},
url = {http://dx.doi.org/10.1039/C3LC41388H},
volume = {13},
year = {2013}
}
TY - JOUR
AB - Measuring protein expression in single cells is the basis of single cell proteomics. The sensitivity and dynamic range of a single cell immunoassay should ideally be such that proteins that are expressed between 1 – 106 copies per cell can be detected and counted. We have investigated the effect of miniaturizing antibody microarrays by reducing capture spot sizes from 100 μm to 15 μm using dip pen nanolithography. We demonstrate that protocols developed for printing and passivating antibody capture spots using conventional pin based contact printing can be directly transferred to dip-pen lithography whilst retaining the capture activity per unit area. Using a simple kinetic model, we highlight how the limit of detection and dynamic range of a sandwich immunoassay, respectively, increase and decrease when spot size is reduced. However, we show that reducing spot size is more effective than increasing assay chamber volume when seeking to multiplex such a microfluidic immunoassay. Although, we make particular reference to single cell microfluidic immunoassays, the topics discussed here are applicable to capture assays in general.
AU - Salehi-Reyhani,A
AU - Sharma,S
AU - Burgin,E
AU - Barclay,M
AU - Cass,AEG
AU - Neil,MEE
AU - Ces,O
AU - Willison,KR
AU - Klug,D
DO - 10.1039/C3LC41388H
EP - 2074
PY - 2013///
SN - 1473-0197
SP - 2066
TI - Scaling Advantages and Constraints in Miniaturized Capture Assays for Single Cell Protein Analysis
T2 - Lab on A Chip
UR - http://dx.doi.org/10.1039/C3LC41388H
UR - http://pubs.rsc.org/en/content/articlelanding/2013/lc/c3lc41388h
VL - 13
ER -
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