LSR II

405nm Violet laser

Collection filterMirrorFluorochromes
450/50 - BV 421, DAPI, Celltrace Violet, CFP, DyLight 405, eFluor 450, Fixable Violet Dead Cell Stain, Fixable Viability eFluor 450,  Hoechst (blue), Pacific Blue
525/50 475LP BV510, AmCyan, BD Horizon V500, Fixable Aqua Dead cell stain, Fixable Viability eFluor 506, Pacific Orange, Qdot 525
605/12 595LP BV605
655/8 635LP BV650
710/50 690LP BV711
780/60 750LP BV786, Qdo t800
Summary of the table's contents

488nm Blue laser

Collection filterMirrorFluorochromes
530/30 505LP FITC, AlexaFluor 488, Calcein, CFSE, Cy2, DyLight 488, DyeCycle Green, eGFP,  eYFP, Fixable green dead cell stain, Fluo-3/Fluo-4, mCitrine, Oyster 488, Rhodamine 110,  Rhodamine 123,  SYTOX Green
575/26 550LP PE, Cy3, pHrodo, SNARF (Low pH)
610/20 600LP PE-Texas Red, PI, PE-CF594, PE-Vio615
660/20 650LP PE-Cy5
695/40 685LP 7-AAD,  PerCP
780/60 755LP  PE-Cy7
Summary of the table's contents
 

633nmRed laser

Collection filterMirrorFluorochromes
660/20 - APC, Alexa Fluor 647, Cell Proliferation Dye eFluor 670, CellVue Maroon, CellVue Plum, Cy5, Cy5.5, DRAQ5, DRAQ7, Dylight 649, Dye Cycle Ruby, Fixable far red dead stain, Fixable Viability eFluor 660, Fx Cycle Far Red Stain, Oyster 650, SYTOX Red, TO-PRO-3, YO-PRO-3
730/45 710LP Alexa Fluor 700, APC-Cy5.5, CellVue Burgundy, DRAQ7, Dye Cycle Ruby
780/60 755LP Alexa Fluor 750, APC-Alexa Fluor 750, APC-Cy7, APC-eFluor 780, APC-H7, APC-Vio770, CellVue NIR780, DRAQ7, Fixable near-IR dead stain,, Fixable Viability eFluor 780
Note: Not every fluorochrome will be able to be used in combination with the ones above, it is the responsibility of the user to research this during panel setup.
Summary of the table's contents

 

National Heart and Lung Institute

General enquiries


St Mary's FACS Facility
Room 312D, third floor
Medical school building 
St Mary's Campus 
Norfolk Place 
Paddington 
London W2 1PG 

Facility Manager
Dr Isabel Correa-Otero
i.correa-otero@imperial.ac.uk