FortessaB laser view

355nm UV laser
Collection filterMirrorFluorochromes
450/50* DAPI, Calcein  Blue AM, Fixable Blue Dead Cell Stain, Indo-1hi, Indo-1 AM, Hoechst 35258, Nuc Blue Live Cell Stain
530/30** 505LP Alexa Fluor 430, Indo-1lo, Qdot 525
*450/50 filter can be exchangeg with 379/28 filter for BUV398; **530/30 filter can be exchanged with 740/35 filter and LP690 filter for BUV737

405nm Violet laser

Collection filterMirrorFluorochromes
450/50 - Alexa Fluor 405, BD Horizon V450, BV421, Calcein Violet, Calcein Violet 450AM, Celltrace Violet, CFP, DAPI, DyLight 405, DyeCycle Violet, eFluor 450, Fixable Violet Dead Cell Stain, Fixable Viability eFluor 450, FxCycle Violet, Hoechst (blue), Pacific Blue, PO-PRO-1, SYTOX Blue, VioBlue
525/50 475LP AmCyan, BD Horizon V500, Brilliant Violet 510, Fixable Aqua Dead cell stain, Fixable Viability eFluor 506, Pacific Orange, Qdot 525
605/12 595LP Brilliant Violet 605, Qdot 605
655/8 630LP Brilliant Violet 650, Qdot 655
710/50 690LP Brilliant Violet 711
780/60 750LP Brilliant Violet 786, Super Bright 780
Summary of the table's contents

488nm Blue laser

Collection filterMirrorFluorochromes
530/30 505LP AlexaFluor 488, Calcein, CFSE, DyLight 488, DyeCycle Green, eGFP,  eYFP , FITC, Fixable green dead cell stain, mCitrine, SYTOX Green, TO-PRO-1, YO-PRO-1
675/20 655LP 7-AAD, PerCP, PI, (PE-Cy5**)
710/40 685LP 7-AAD, PerCP-Cy5.5, PerCP-eFluor710 

**PE-Cy5 is not recommended for use with APC

Summary of the table's contents

561nm Yellow Green laser

Collection filterMirrorFluorochromes
582/15 570LP PE, DsRed, tdTomato, RFP
620/10 600LP m-Cherry, PE-Texas Red, PI, Texas Red, PE/Dazzle 594, PE-CF594, PE-eFluor® 610, and ECD
710/50 685LP 7-AAD, Pe-Cy5.5
780/60 750LP PE-Cy7, PE-Vio 770
Summary of the table's contents

640nm Red laser

Collection filterMirrorFluorochromes
670/14 - APC, Alexa Fluor 647, Cell Proliferation Dye eFluor 670, Cy5, Cy5.5, DRAQ5, DRAQ7, Dylight 649, Dye Cycle Ruby, Fixable far red dead stain, Fixable Viability eFluor 660, Fx Cycle Far Red Stain, Oyster 650, SYTOX Red, TO-PRO-3, YO-PRO-3
730/45 710LP Alexa Fluor 700, APC-Cy5.5,  DRAQ7, Dye Cycle Ruby
780/60 750LP Alexa Fluor 750, APC-Alexa Fluor 750, APC-Cy7, APC-eFluor 780, APC-H7, APC-Vio770, Fixable near-IR dead stain,, Fixable Viability eFluor 780
Note: Not every fluorochrome will be able to be used in combination with the ones above, it is the responsibility of the user to research this during panel setup.
Summary of the table's contents

 

National Heart and Lung Institute

General enquiries


St Mary's FACS Facility
Room 312D, third floor
Medical school building 
St Mary's Campus 
Norfolk Place 
Paddington 
London W2 1PG 

Facility Manager
Dr Isabel Correa-Otero
i.correa-otero@imperial.ac.uk