405nm Violet
| Collection filter | Mirror | Fluorochromes |
|---|---|---|
| 450/50 | - | Alexa Fluor 405, BD Horizon V450, BV 421, Calcein Violet, Calcein Violet 450AM, Celltrace Violet, CFP, DAPI, DyLight 405, DyeCycle Violet, eFluor 450, Fixable Violet Dead Cell Stain, Fixable Viability eFluor 450, FxCycle Violet, Hoechst (blue), Pacific Blue, PO-PRO-1, SYTOX Blue, VioBlue |
| 525/50 | 475LP | AmCyan, BD Horizon V500, Brilliant Violet 510, Fixable Aqua Dead cell stain, Fixable Viability eFluor 506, Pacific Orange, Qdot 525, VioGreen |
| 605/12 | 595LP | BV605 |
| 660/40 | 630LP | BV650 |
| 720/20 | 700LP | BV711 |
| 780/60 | 735LP | BV786, Qdot 800 |
488nm Blue
| Collection filter | Mirror | Fluorochromes |
|---|---|---|
| 525/50 | 505LP | AlexaFluor 488, Calcein, CellVue Jade, CFSE, Cy2, DyLight 488, DyeCycle Green, eGFP, eYFP , FITC, Fixable green dead cell stain, SYTOX Green, TO-PRO-1, YO-PRO-1 |
| 710/50 | 685LP | 7-AAD, PerCP-Cy5.5, PerCP-eFluor710 |
561nm Yellow Green
| Collection filter | Mirror | Fluorochromes |
|---|---|---|
| 582/15 | 570LP | PE, DsRed, tdTomato, RFP |
| 620/10 | 600LP | m-Cherry, PE-Texas Red, PI, PE-CF594, Texas Red |
| 710/50 | 685LP | 7-AAD, Pe-Cy5.5, PI |
| 780/60 | 755LP | PE-Cy7, PE-Vio 770 |
633nm Red
| Collection filter | Mirror | Fluorochromes |
|---|---|---|
| 670/30 | - | APC, Alexa Fluor 647, Cell Proliferation Dye eFluor 670, Cy5.5, DRAQ5, DRAQ7, Dylight 649, Fixable far red dead stain, Fixable Viability eFluor 660, Fx Cycle Far Red Stain, Oyster 650, SYTOX Red, TO-PRO-3, YO-PRO-3 |
| 730/45 | 685LP | Alexa Fluor 700, APC-Cy5.5, DRAQ7, Dye Cycle Ruby |
| 780/60 | 755LP | Alexa Fluor 750, APC-Alexa Fluor 750, APC-Cy7, APC-eFluor 780, APC-H7, APC-Vio770, CellVue NIR780, DRAQ7, Fixable near-IR dead stain,, Fixable Viability eFluor 780 |
| Note: Not every fluorochrome will be able to be used in combination with the ones above, it is the responsibility of the user to research this during panel setup. | ||
Useful links
General enquiries
St Mary's FACS Facility
Room 312D, third floor
Medical school building
St Mary's Campus
Norfolk Place
Paddington
London W2 1PG
Facility Manager
Dr Isabel Correa-Otero
i.correa-otero@imperial.ac.uk