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@article{Taylor:2018:10.1016/j.ijantimicag.2018.03.016,
author = {Taylor, E and Sriskandan, S and Woodford, N and Hopkins, K},
doi = {10.1016/j.ijantimicag.2018.03.016},
journal = {International Journal of Antimicrobial Agents},
pages = {278--282},
title = {High prevalence of 16S rRNA methyltransferases among carbapenemase-producing Enterobacteriaceae in the UK & Ireland},
url = {http://dx.doi.org/10.1016/j.ijantimicag.2018.03.016},
volume = {52},
year = {2018}
}
TY - JOUR
AB - The emergence of 16S rRNA methyltransferases (16S RMTases) worldwide is a growing concern due to their ability to confer high-level resistance (MICs >256 mg/L) to all clinically-relevant aminoglycosides. As the occurrence of 16S RMTases in the United Kingdom has not been investigated to date, we screened 806 Enterobacteriaceae isolates displaying high-level aminoglycoside resistance (amikacin, gentamicin and tobramycin MICs ≥64, ≥32 and ≥32 mg/L, respectively) for 16S RMTases either by analysing whole-genome sequence (WGS) data (which were available for 449 isolates) or by PCR. A total of 94.5% (762/806) pan-aminoglycoside resistant Enterobacteriaceae were positive for one or more 16S RMTase genes; armA was the most common (340, 44.6%) followed by rmtC (146, 19.2%), rmtF (137, 18.0%), rmtB (87, 11.4%) and various two gene combinations (52, 6.8%). Most (93.4%; 712/762) 16S RMTase producers also carried acquired carbapenemase genes, with blaNDM the most common (592/712; 83.1%). Additionally, high-risk bacterial clones associated with blaNDM were identified in the subset of isolates with WGS data. These included E. coli sequence types (STs) 405 [21.8%, 19/87], 167 [20.7%, 18/87] 410 [12.6%, 11/87] and K. pneumoniae STs 14 [35.6%, 112/315], 231 [15.6%, 49/315] and 147 [10.5%, 33/315]. These accounted for 4.2% [15/358], 5.0% [18/358], 3.1% [11/358], 28.2% [101/358], 3.1% [11/358] and 7.0% [25/358] blaNDM-producing isolates, respectively. This study shows that 16S RMTases occur in the UK & Ireland and carbapenemases are particularly prevalent in 16S RMTase-producing Enterobacteriaceae. This association poses a risk to the treatment of multidrug-resistant Gram-negative infections in the clinical setting.
AU - Taylor,E
AU - Sriskandan,S
AU - Woodford,N
AU - Hopkins,K
DO - 10.1016/j.ijantimicag.2018.03.016
EP - 282
PY - 2018///
SN - 0924-8579
SP - 278
TI - High prevalence of 16S rRNA methyltransferases among carbapenemase-producing Enterobacteriaceae in the UK & Ireland
T2 - International Journal of Antimicrobial Agents
UR - http://dx.doi.org/10.1016/j.ijantimicag.2018.03.016
UR - http://hdl.handle.net/10044/1/58457
VL - 52
ER -
