BibTex format
@article{Afshar:2017:10.1080/21505594.2017.1325070,
author = {Afshar, B and Turner, CE and Lamagni, TL and Smith, K and Al-Shahib, A and Underwood, A and Holden, MTG and Efstratiou, A and Sriskandan, S},
doi = {10.1080/21505594.2017.1325070},
journal = {Virulence},
pages = {1390--1400},
title = {Enhanced nasopharyngeal infection and shedding associated with an epidemic lineage of emm3 group A Streptococcus},
url = {http://dx.doi.org/10.1080/21505594.2017.1325070},
volume = {8},
year = {2017}
}
RIS format (EndNote, RefMan)
TY - JOUR
AB - Background: A group A Streptococcus (GAS) lineage of genotype emm3, sequence type 15 (ST15) was associated with a 6 month upsurge in invasive GAS disease in the UK. The epidemic lineage (Lineage C) had lost 2 typical emm3 prophages, Φ315.1 and Φ315.2 associated with the superantigen ssa, but gained a different prophage (ΦUK-M3.1) associated with a different superantigen, speC and a DNAse spd1. Methods and Results: The presence of speC and spd1 in Lineage C ST15 strains enhanced both in vitro mitogenic and DNase activities over non-Lineage C ST15 strains. Invasive disease models in Galleria mellonella and SPEC-sensitive transgenic mice, revealed no difference in overall invasiveness of Lineage C ST15 strains compared with non-Lineage C ST15 strains, consistent with clinical and epidemiological analysis. Lineage C strains did however markedly prolong murine nasal infection with enhanced nasal and airborne shedding compared with non-Lineage C strains. Deletion of speC or spd1 in 2 Lineage C strains identified a possible role for spd1 in airborne shedding from the murine nasopharynx. Conclusions: Nasopharyngeal infection and shedding of Lineage C strains was enhanced compared with non-Lineage C strains and this was, in part, mediated by the gain of the DNase spd1 through prophage acquisition.
AU - Afshar,B
AU - Turner,CE
AU - Lamagni,TL
AU - Smith,K
AU - Al-Shahib,A
AU - Underwood,A
AU - Holden,MTG
AU - Efstratiou,A
AU - Sriskandan,S
DO - 10.1080/21505594.2017.1325070
EP - 1400
PY - 2017///
SN - 2150-5608
SP - 1390
TI - Enhanced nasopharyngeal infection and shedding associated with an epidemic lineage of emm3 group A Streptococcus
T2 - Virulence
UR - http://dx.doi.org/10.1080/21505594.2017.1325070
UR - http://hdl.handle.net/10044/1/48335
VL - 8
ER -
