In this section
@article{Allgöwer:2024:10.1073/pnas.2413396121,
author = {Allgöwer, F and Pöverlein, MC and Rutherford, AW and Kaila, VRI},
doi = {10.1073/pnas.2413396121},
journal = {Proceedings of the National Academy of Sciences},
title = {Mechanism of proton release during water oxidation in Photosystem II},
url = {http://dx.doi.org/10.1073/pnas.2413396121},
volume = {121},
year = {2024}
}
TY - JOUR
AB - <jats:p> Photosystem II (PSII) catalyzes light-driven water oxidation that releases dioxygen into our atmosphere and provides the electrons needed for the synthesis of biomass. The catalysis occurs in the oxygen-evolving oxo-manganese-calcium (Mn <jats:sub>4</jats:sub> O <jats:sub>5</jats:sub> Ca) cluster that drives the oxidation and deprotonation of substrate water molecules leading to the O <jats:sub>2</jats:sub> formation. However, despite recent advances, the mechanism of these reactions remains unclear and much debated. Here, we show that the light-driven Tyr161 <jats:sub>D1</jats:sub> (Y <jats:sub>z</jats:sub> ) oxidation adjacent to the Mn <jats:sub>4</jats:sub> O <jats:sub>5</jats:sub> Ca cluster, decreases the barrier for proton transfer from the putative substrate water molecule (W3/W <jats:sub>x</jats:sub> ) to Glu310 <jats:sub>D2</jats:sub> , accessible to the luminal bulk. By combining hybrid quantum/classical (QM/MM) free energy calculations with atomistic molecular dynamics simulations, we probe the energetics of the proton transfer along the Cl1 pathway. We demonstrate that the proton transfer occurs via water molecules and a cluster of conserved carboxylates, driven by redox-triggered electric fields directed along the pathway. Glu65 <jats:sub>D1</jats:sub> establishes a local molecular gate that controls the proton transfer to the luminal bulk, while Glu312 <jats:sub>D2</jats:sub> acts as a local proton storage site. The identified gating region could be important in preventing backflow of protons to the Mn <jats:sub>4</jats:sub>
AU - Allgöwer,F
AU - Pöverlein,MC
AU - Rutherford,AW
AU - Kaila,VRI
DO - 10.1073/pnas.2413396121
PY - 2024///
SN - 0027-8424
TI - Mechanism of proton release during water oxidation in Photosystem II
T2 - Proceedings of the National Academy of Sciences
UR - http://dx.doi.org/10.1073/pnas.2413396121
UR - https://doi.org/10.1073/pnas.2413396121
VL - 121
ER -
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