BibTex format
@article{Selvaraj:2024:10.1371/journal.pbio.3002868,
author = {Selvaraj, M and Toghani, A and Pai, H and Sugihara, Y and Kourelis, J and Yuen, ELH and Ibrahim, T and Zhao, H and Xie, R and Maqbool, A and De, la Concepcion JC and Banfield, MJ and Derevnina, L and Petre, B and Lawson, DM and Bozkurt, TO and Wu, C-H and Kamoun, S and Contreras, MP},
doi = {10.1371/journal.pbio.3002868},
journal = {PLoS Biology},
title = {Activation of plant immunity through conversion of a helper NLR homodimer into a resistosome},
url = {http://dx.doi.org/10.1371/journal.pbio.3002868},
year = {2024}
}
RIS format (EndNote, RefMan)
TY - JOUR
AB - Nucleotide-binding domain and leucine-rich repeat (NLR) proteins can engage in complex interactions to detect pathogens and execute a robust immune response via downstream helper NLRs. However, the biochemical mechanisms of helper NLR activation by upstream sensor NLRs remain poorly understood. Here, we show that the coiled-coil helper NLR NRC2 from Nicotiana benthamiana accumulates in vivo as a homodimer that converts into a higher-order oligomer upon activation by its upstream virus disease resistance protein Rx. The cryo-EM structure of NbNRC2 in its resting state revealed intermolecular interactions that mediate homodimer formation and contribute to immune receptor autoinhibition. These dimerization interfaces have diverged between paralogous NRC proteins to insulate critical network nodes and enable redundant immune pathways, possibly to minimise undesired cross-activation and evade pathogen suppression of immunity. Our results expand the molecular mechanisms of NLR activation pointing to transition from homodimers to higher-order oligomeric resistosomes.
AU - Selvaraj,M
AU - Toghani,A
AU - Pai,H
AU - Sugihara,Y
AU - Kourelis,J
AU - Yuen,ELH
AU - Ibrahim,T
AU - Zhao,H
AU - Xie,R
AU - Maqbool,A
AU - De,la Concepcion JC
AU - Banfield,MJ
AU - Derevnina,L
AU - Petre,B
AU - Lawson,DM
AU - Bozkurt,TO
AU - Wu,C-H
AU - Kamoun,S
AU - Contreras,MP
DO - 10.1371/journal.pbio.3002868
PY - 2024///
SN - 1544-9173
TI - Activation of plant immunity through conversion of a helper NLR homodimer into a resistosome
T2 - PLoS Biology
UR - http://dx.doi.org/10.1371/journal.pbio.3002868
UR - http://hdl.handle.net/10044/1/115334
ER -