In this section
@article{Marshall:2024,
author = {Marshall, EKP and Nunes, C and Burbaud, S and Vincent, CM and Munroe, NO and Simoes, da Silva CJ and Wadhawan, A and Pearson, WH and Sangen, J and Boeck, L and Floto, RA and Dionne, M},
journal = {Proceedings of the National Academy of Sciences of USA},
title = {Microbial metabolism disrupts cytokine activity to impact host immune response},
url = {http://hdl.handle.net/10044/1/115176},
year = {2024}
}
TY - JOUR
AB - Host-pathogen interactions are shaped by the metabolic status of both the host and pathogen. The host must regulate metabolism to fuel the immune response, whilst the pathogen must extract metabolic resources from the host to enable its own survival. In this study, we focus on the metabolic interactions of Mycobacterium abscessus with Drosophila melanogaster. We identify MAB_1132 as an asparagine transporter required for pathogenicity in M. abscessus. We show that this requirement is specifically associated with damage to the host: flies infected with MAB_1132c knockout bacteria, or with wild-type bacteria grown in asparagine-restricted conditions, are longer lived without showing a significant change in bacterial load. This is associated with a reduction in the host innate immune response, demonstrated by the decreased transcription of antimicrobial peptides, as well as a significant reduction in the ability of the infection to disrupt systemic insulin signalling. Much of the increase in host survival during infection with asparagine-limited M. abscessus can be attributed to alterations in unpaired cytokine signalling. This demonstrates that asparagine transport in M. abscessus prior to infection is not required for replicative fitness in vivo but does significantly influence the interaction with the host immune responses.
AU - Marshall,EKP
AU - Nunes,C
AU - Burbaud,S
AU - Vincent,CM
AU - Munroe,NO
AU - Simoes,da Silva CJ
AU - Wadhawan,A
AU - Pearson,WH
AU - Sangen,J
AU - Boeck,L
AU - Floto,RA
AU - Dionne,M
PY - 2024///
SN - 0027-8424
TI - Microbial metabolism disrupts cytokine activity to impact host immune response
T2 - Proceedings of the National Academy of Sciences of USA
UR - http://hdl.handle.net/10044/1/115176
ER -
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