The BD FACSAria Fusion is offered for CL2 work with cultured cells and patient samples only. For work with bacteria and yeast please consult our other cell sorters. Please refer to the Biosafety page (link at the bottom of this page) for more information on the documentation needed to use this instrument.

The FACSAria Fusion is a 14-colour high speed cell sorter with three lasers (Violet 405nm, Blue 488nm and Red 640nm), an integrated biosafety cabinet, a fixed optical alignment and a pressure-driven fluidics system. The flow cell is a gel-coupled cuvette as opposed to the jet-in-air system, and this combined with octagon detection systems provide increased sensitivity and resolution. This arrangement allows filter and mirror changes within the optical array to be made easily and requires no additional alignment for maximum signal strength. To further enhance biosafety protection, the BD FACSAria Fusion has an integrated biosafety cabinet a built-in Aerosol Management System (AMS) that evacuates aerosols.

A choice of nozzles lets users sort a wide range of cell sizes. It is equipped with a versatile sort collection chamber that allows users to sort into tubes, slides and plates. Cooling and heating for sort collection are available.

Forward and side scatter properties (FSC and SSC) allow for a minimum detectable particle size of 0.5 μm. The maximum data acquisition rate is 70,000 events/second. We recommend a minimum sample volume of 500 μL.

For more information, please read the BD FACSAria Fusion technical specifications‌.

Download a complete configuration of the Fusion Lasers and Filters‌, including fluorochromes and bandpass filters. You can also find it below.

Violet 405nm
Bandpass filterMirrorFluorochrome
 450/50  -   DAPI, Pacific Blue, BV421, V450 
 525/50  505LP   AmCyan, BV510, V500
 605/20  595LP  BV605
 660/20  630LP   BV650
 710/50  690LP   BV711
 780/60  750LP  BV786
Summary of the table's contents
Blue 488nm
Bandpass filterMirrorFluorochrome
 488/15  -   -
 530/30  502LP   FITC, AF488, GFP, BV515
 585/42  556LP  PE, PI
 610/20  610LP  PE-CF594
 695/40  655LP  PerCP-Cy5.5, Per-CP, 7-AAD
 780/60  735LP  PE-Cy7
Summary of the table's contents
Red 640nm
Bandpass filterMirrorFluorochrome
 670/20  -  APC, AF647
 730/45  690LP  AF700
 780/60  755LP  APC-Cy7, APC-H7
Summary of the table's contents

Location

The Royal Brompton hospital, Guy Scadding building. Please book via the St Mary’s Flow Facility PPMS.

The Fusion is available to any trained user 24 hours a day (subject to building access and regulations).

General enquiries


Flow Cytometry Facility
South Kensington
Sir Alexander Fleming Building
Fifth Floor, Room 532
South Kensington Campus
Imperial College London
Exhibition Road
London SW7 2AZ, UK

flow@imperial.ac.uk
+44 (0)20 7594 5423