BD LSRFortessa II

The BD LSRFortessa II is offered for CL2 work. Please refer to the Biosafety page (link at the bottom of this page) for more information on the documentation needed to use this instrument.

The Fortessa II is a 14 colour analyser with four lasers (Violet 405nm, Blue 488nm, Yellow-Green 561nm and Red 640nm), a fixed optical alignment and a pressure-driven fluidics system. The flow cell is a gel-coupled cuvette as opposed to the jet-in-air system, and this combined with trigon and octagon detection systems provide increased sensitivity and resolution. This arrangement allows filter and mirror changes within the optical array to be made easily and requires no additional alignment for maximum signal strength.

It is equipped with an HTS (High Throughput System) or plate reader that is capable of reading from 96 or 384 well plates, as well as from tubes.

Forward and side scatter properties (FSC and SSC) allow for a minimum detectable particle size of 0.5 μm. The maximum data acquisition rate is 40,000 events/second. We recommend a minimum sample volume of 300 μL in standard mode and 200 μL in HTS mode.

For more information, please read the BD LSRFortessa Cell Analyzer Technical Specifications and BD HTS Option.

Download a complete configuration of Fortessa II Lasers and Filters, including fluorochromes and bandpass filters. You can also find it below.

Location

Sir Alexander Fleming Building, 6^th floor, Room 638.

The Fortessa II is available to any trained user 24 hours a day (subject to building access and regulations).