In this section
@article{Rajakumar:2019:10.1177/2472630318798304,
author = {Rajakumar, PD and Gower, G and Suckling, L and Kitney, R and McClymont, D and Freemont, P},
doi = {10.1177/2472630318798304},
journal = {Slas Technology},
pages = {291--297},
title = {Rapid prototyping platform for Saccharomyces cerevisiae using computer-aided genetic design enabled by parallel software and workcell platform development},
url = {http://dx.doi.org/10.1177/2472630318798304},
volume = {24},
year = {2019}
}
TY - JOUR
AB - Biofoundries have enabled the ability to automate the construction of genetic constructs using computer-aided design. In this study, we have developed the methodology required to abstract and automate the construction of yeast-compatible designs. We demonstrate the use of our in-house software tool, AMOS, to coordinate with design software, JMP, and robotic liquid handling platforms to successfully manage the construction of a library of 88 yeast expression plasmids. In this proof-of-principle study, we used three fluorescent genes as proxy for three enzyme coding sequences. Our platform has been designed to quickly iterate around a design cycle of four protein coding sequences per plasmid, with larger numbers possible with multiplexed genome integrations in Saccharomyces cerevisiae. This work highlights how developing scalable new biotechnology applications requires a close integration between software development, liquid handling robotics, and protocol development.
AU - Rajakumar,PD
AU - Gower,G
AU - Suckling,L
AU - Kitney,R
AU - McClymont,D
AU - Freemont,P
DO - 10.1177/2472630318798304
EP - 297
PY - 2019///
SN - 2472-6303
SP - 291
TI - Rapid prototyping platform for Saccharomyces cerevisiae using computer-aided genetic design enabled by parallel software and workcell platform development
T2 - Slas Technology
UR - http://dx.doi.org/10.1177/2472630318798304
UR - http://hdl.handle.net/10044/1/63436
VL - 24
ER -
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