In this section
@article{Watson and Andrews:2017:10.1371/journal.pone.0180309,
author = {Watson and Andrews, N and Davis, S and Bugeon, L and Dallman, M and McGinty, J},
doi = {10.1371/journal.pone.0180309},
journal = {PLOS One},
title = {OPTiM: optical projection tomography integrated microscope using open-source hardware and software},
url = {http://dx.doi.org/10.1371/journal.pone.0180309},
volume = {12},
year = {2017}
}
TY - JOUR
AB - We describe the implementation of an OPT plate to perform optical projection tomography (OPT) on a commercial wide-field inverted microscope, using our open-source hardware and software. The OPT plate includes a tilt adjustment for alignment and a stepper motor for sample rotation as required by standard projection tomography. Depending on magnification requirements, three methods of performing OPT are detailed using this adaptor plate: a conventional direct OPT method requiring only the addition of a limiting aperture behind the objective lens; an external optical-relay method allowing conventional OPT to be performed at magnifications >4x; a remote focal scanning and region-of-interest method for improved spatial resolution OPT (up to ~1.6 μm). All three methods use the microscope’s existing incoherent light source (i.e. arc-lamp) and all of its inherent functionality is maintained for day-to-day use. OPT acquisitions are performed on in vivo zebrafish embryos to demonstrate the implementations’ viability.
AU - Watson
AU - Andrews,N
AU - Davis,S
AU - Bugeon,L
AU - Dallman,M
AU - McGinty,J
DO - 10.1371/journal.pone.0180309
PY - 2017///
SN - 1932-6203
TI - OPTiM: optical projection tomography integrated microscope using open-source hardware and software
T2 - PLOS One
UR - http://dx.doi.org/10.1371/journal.pone.0180309
UR - http://hdl.handle.net/10044/1/49603
VL - 12
ER -