In this section
@article{Duarte:2019:10.1111/imcb.12216,
author = {Duarte, D and Amarteifio, S and Ang, H and Kong, IY and Ruivo, N and Pruessner, G and Hawkins, ED and Lo, Celso C},
doi = {10.1111/imcb.12216},
journal = {Immunology and Cell Biology},
pages = {229--235},
title = {Defining the in vivo characteristics of acute myeloid leukemia cells behavior by intravital imaging},
url = {http://dx.doi.org/10.1111/imcb.12216},
volume = {97},
year = {2019}
}
TY - JOUR
AB - The majority of acute myeloid leukemia (AML) patients have a poor response to conventional chemotherapy. The survival of chemoresistant cells is thought to depend on leukemia-bone marrow (BM) microenvironment interactions, which are not well understood. The CXCL12/CXCR4 axis has been proposed to support AML growth but was not studied at the single AML cell level. We recently showed that T-cell acute lymphoblastic leukemia (T-ALL) cells are highly motile in the BM; however, the characteristics of AML cell migration within the BM remain undefined. Here, we characterize the in vivo migratory behavior of AML cells and their response to chemotherapy and CXCR4 antagonism, using high-resolution 2-photon and confocal intravital microscopy of mouse calvarium BM and the well-established MLL-AF9-driven AML mouse model. We used the Notch1-driven T-ALL model as a benchmark comparison and AMD3100 for CXCR4 antagonism experiments. We show that AML cells are migratory, and in contrast with T-ALL, chemoresistant AML cells become less motile. Moreover, and in contrast with T-ALL, the in vivo exploratory behavior of expanding and chemoresistant AML cells is unaffected by AMD3100. These results expand our understanding of AML cells-BM microenvironment interactions, highlighting unique traits of leukemia of different lineages.
AU - Duarte,D
AU - Amarteifio,S
AU - Ang,H
AU - Kong,IY
AU - Ruivo,N
AU - Pruessner,G
AU - Hawkins,ED
AU - Lo,Celso C
DO - 10.1111/imcb.12216
EP - 235
PY - 2019///
SN - 0818-9641
SP - 229
TI - Defining the in vivo characteristics of acute myeloid leukemia cells behavior by intravital imaging
T2 - Immunology and Cell Biology
UR - http://dx.doi.org/10.1111/imcb.12216
UR - https://www.ncbi.nlm.nih.gov/pubmed/30422351
UR - http://hdl.handle.net/10044/1/66316
VL - 97
ER -