In this section
@article{Loftus:2018:10.1021/acs.nanolett.8b01089,
author = {Loftus, C and Saeed, M and Davis, D and Dunlop, IE},
doi = {10.1021/acs.nanolett.8b01089},
journal = {Nano Letters: a journal dedicated to nanoscience and nanotechnology},
pages = {3282--3289},
title = {Activation of human Natural Killer cells by graphene oxide-templated antibody nanoclusters},
url = {http://dx.doi.org/10.1021/acs.nanolett.8b01089},
volume = {18},
year = {2018}
}
TY - JOUR
AB - An emerging new paradigm is that immune cell activation is controlled by transient interactions between supramolecular assemblies of receptors and ligands. Current immunotherapy biologic pharmaceuticals that activate or desensitize NK cells are, however, individual molecules that do not replicate this nanoscale organization of proteins. Here, we use nanoscale graphene oxide (NGO) as a template to generate soluble nanoscale clusters of Natural Killer cell-activating antibodies. We control nanocluster size and molecular number to mimic reported values for cell surface proteins. These NGO-templated molecular nanoclusters, used to stimulate NK cells via the CD16 receptor, successfully induced cellular activation, indicated by degranulation of cytolytic granules and IFN-γ secretion. Importantly, activation significantly exceeded that induced by the same antibodies applied as a solution of individual molecules. These results demonstrate that future immunotherapies could be enhanced by assembling immunomodulatory drugs into nanoclusters and establish NGO-templating as a candidate technology.
AU - Loftus,C
AU - Saeed,M
AU - Davis,D
AU - Dunlop,IE
DO - 10.1021/acs.nanolett.8b01089
EP - 3289
PY - 2018///
SN - 1530-6984
SP - 3282
TI - Activation of human Natural Killer cells by graphene oxide-templated antibody nanoclusters
T2 - Nano Letters: a journal dedicated to nanoscience and nanotechnology
UR - http://dx.doi.org/10.1021/acs.nanolett.8b01089
UR - https://pubs.acs.org/doi/10.1021/acs.nanolett.8b01089
UR - http://hdl.handle.net/10044/1/59111
VL - 18
ER -
