In this section
@article{Magness:2017:2057-1739/aa6aae,
author = {Magness, AJ and Squires, J and Griffiths, B and Khan, K and Swain, A and Willison, K and Cunningham, D and Gerlinger, M and Klug, D},
doi = {2057-1739/aa6aae},
journal = {Convergent Science Physical Oncology},
title = {Multiplexed single cell protein expression analysis in solid tumours using a miniaturised microfluidic assay},
url = {http://dx.doi.org/10.1088/2057-1739/aa6aae},
volume = {3},
year = {2017}
}
TY - JOUR
AB - Using patient-derived colorectal cancer xenografts, we demonstrate a practicable workflow for single cell proteomics in clinically relevant samples and thus a potential translational route for single cell proteomics into medical diagnostics. Using a microfluidic antibody capture [MAC] chip we measured the expression of the tumour suppressor protein p53 and of its post-translationally modified form phosphorylated at serine-15. Aberrant expression of these has commonly been found in colorectal cancers and has been widely investigated for prognostic significance. Our results show that the MAC technology is viable for quantitatively assessing protein expression and phosphorylation at the single cell level in microscopic amounts of clinically relevant tumour material. Thus, this could become a useful tool in therapeutic-associated single cell protein analysis. We also found dramatic variability of p53 and phosphorylated p53 quantities between individual cancer cells from the same sample, demonstrating the power of this single cell technology to study functional intratumour heterogeneity.
AU - Magness,AJ
AU - Squires,J
AU - Griffiths,B
AU - Khan,K
AU - Swain,A
AU - Willison,K
AU - Cunningham,D
AU - Gerlinger,M
AU - Klug,D
DO - 2057-1739/aa6aae
PY - 2017///
SN - 2057-1739
TI - Multiplexed single cell protein expression analysis in solid tumours using a miniaturised microfluidic assay
T2 - Convergent Science Physical Oncology
UR - http://dx.doi.org/10.1088/2057-1739/aa6aae
UR - http://hdl.handle.net/10044/1/46253
VL - 3
ER -
