Publications

Citation

BibTex format

@article{Beckova:2017:10.1098/rstb.2016.0394,
author = {Beckova, M and Yu, J and Krynicka, V and Kozlo, A and Shao, S and Konik, P and Komenda, J and Murray, JW and Nixon, PJ},
doi = {10.1098/rstb.2016.0394},
journal = {Philosophical Transactions of the Royal Society B: Biological Sciences},
title = {Structure of Psb29/Thf1 and its association with the FtsH protease complex involved in photosystem II repair in cyanobacteria},
url = {http://dx.doi.org/10.1098/rstb.2016.0394},
volume = {372},
year = {2017}
}

Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - One strategy for enhancing photosynthesis in crop plants is to improve the ability to repair photosystem II (PSII) in response to irreversible damage by light. Despite the pivotal role of thylakoid embedded FtsH protease complexes in the selective degradation of PSII subunits during repair, little is known about the factors involved in regulating FtsH expression. Here we show using the cyanobacterium Synechocystis sp. PCC 6803 that the Psb29 subunit, originally identified as a minor component of His tagged PSII preparations, physically interacts with FtsH complexes in vivo and is required for normal accumulation of the FtsH2/FtsH3 hetero oligomeric complex involved in PSII repair. We show using X ray crystallography that Psb29 from Thermosynechococcus elongatushas a unique fold consisting of a helical bundle and an extended C terminal helix and contains a highly conserved region that might be involved in binding to FtsH. A similar interaction is likely to occur in Arabidopsis chloroplasts between the Psb29 homologue, termed THF1, and the FTSH2/FTSH5 complex. The direct involvement of Psb29/THF1 in FtsH accumulation helps explain why THF1 is a target during the hypersensitive response in plants induced by pathogen infection. Downregulating FtsH function and the PSII repair cycle via THF1 would contribute to the production
AU  - Beckova,M
AU  - Yu,J
AU  - Krynicka,V
AU  - Kozlo,A
AU  - Shao,S
AU  - Konik,P
AU  - Komenda,J
AU  - Murray,JW
AU  - Nixon,PJ
DO  - 10.1098/rstb.2016.0394
PY  - 2017///
SN  - 1471-2970
TI  - Structure of Psb29/Thf1 and its association with the FtsH protease complex involved in photosystem II repair in cyanobacteria
T2  - Philosophical Transactions of the Royal Society B: Biological Sciences
UR  - http://dx.doi.org/10.1098/rstb.2016.0394
UR  - http://hdl.handle.net/10044/1/44306
VL  - 372
ER  -

Download

Citation

BibTex format

RIS format (EndNote, RefMan)

Centre for Structural Biology Open Day

Useful Links

Structural Biology