BibTex format
@article{Rutherford:2016:10.1038/srep20086,
author = {Rutherford, AW and Prell, J and MacKellar, D and Tobin, C and Lieber, L and Freisen, M and Norman, JS and Bolger, A and Oksaksin, M and Chang, RL and Ford, TL and Nguyen, PQ and Woodward, J and Permingeat, HR and Joshi, NS and Silver, PA and Usadel, B and Murray, JW},
doi = {10.1038/srep20086},
journal = {Scientific Reports},
title = {Streptomyces thermoautotrophicus does not fix nitrogen},
url = {http://dx.doi.org/10.1038/srep20086},
volume = {6},
year = {2016}
}
RIS format (EndNote, RefMan)
TY - JOUR
AB - Streptomyces thermoautotrophicus UBT1 has been described as a moderately thermophilic chemolithoautotroph with a novel nitrogenase enzyme that is oxygen-insensitive. We have cultured the UBT1 strain, and have isolated two new strains (H1 and P1-2) of very similar phenotypic and genetic characters. These strains show minimal growth on ammonium-free media, and fail to incorporate isotopically labeled N2 gas into biomass in multiple independent assays. The sdn genes previously published as the putative nitrogenase of S. thermoautotrophicus have little similarity to anything found in draft genome sequences, published here, for strains H1 and UBT1, but share >99% nucleotide identity with genes from Hydrogenibacillus schlegelii, a draft genome for which is also presented here. H. schlegelii similarly lacks nitrogenase genes and is a non-diazotroph. We propose reclassification of the species containing strains UBT1, H1, and P1-2 as a non-Streptomycete, non-diazotrophic, facultative chemolithoautotroph and conclude that the existence of the previously proposed oxygen-tolerant nitrogenase is extremely unlikely.
AU - Rutherford,AW
AU - Prell,J
AU - MacKellar,D
AU - Tobin,C
AU - Lieber,L
AU - Freisen,M
AU - Norman,JS
AU - Bolger,A
AU - Oksaksin,M
AU - Chang,RL
AU - Ford,TL
AU - Nguyen,PQ
AU - Woodward,J
AU - Permingeat,HR
AU - Joshi,NS
AU - Silver,PA
AU - Usadel,B
AU - Murray,JW
DO - 10.1038/srep20086
PY - 2016///
SN - 2045-2322
TI - Streptomyces thermoautotrophicus does not fix nitrogen
T2 - Scientific Reports
UR - http://dx.doi.org/10.1038/srep20086
UR - http://hdl.handle.net/10044/1/29115
VL - 6
ER -
