BibTex format
@article{Tenboer:2014:10.1126/science.1259357,
author = {Tenboer, J and Basu, S and Zatsepin, N and Pande, K and Milathianaki, D and Frank, M and Hunter, M and Boutet, S and Williams, GJ and Koglin, JE and Oberthuer, D and Heymann, M and Kupitz, C and Conrad, C and Coe, J and Roy-Chowdhury, S and Weierstall, U and James, D and Wang, D and Grant, T and Barty, A and Yefanov, O and Scales, J and Gati, C and Seuring, C and Srajer, V and Henning, R and Schwander, P and Fromme, R and Ourmazd, A and Moffat, K and Van, Thor JJ and Spence, JCH and Fromme, P and Chapman, HN and Schmidt, M},
doi = {10.1126/science.1259357},
journal = {Science},
pages = {1242--1246},
title = {Time-resolved serial crystallography captures high-resolution intermediates of photoactive yellow protein},
url = {http://dx.doi.org/10.1126/science.1259357},
volume = {346},
year = {2014}
}
RIS format (EndNote, RefMan)
TY - JOUR
AB - Serial femtosecond crystallography using ultrashort pulses from x-ray free electron lasers (XFELs) enables studies of the light-triggered dynamics of biomolecules. We used microcrystals of photoactive yellow protein (a bacterial blue light photoreceptor) as a model system and obtained high-resolution, time-resolved difference electron density maps of excellent quality with strong features; these allowed the determination of structures of reaction intermediates to a resolution of 1.6 angstroms. Our results open the way to the study of reversible and nonreversible biological reactions on time scales as short as femtoseconds under conditions that maximize the extent of reaction initiation throughout the crystal.
AU - Tenboer,J
AU - Basu,S
AU - Zatsepin,N
AU - Pande,K
AU - Milathianaki,D
AU - Frank,M
AU - Hunter,M
AU - Boutet,S
AU - Williams,GJ
AU - Koglin,JE
AU - Oberthuer,D
AU - Heymann,M
AU - Kupitz,C
AU - Conrad,C
AU - Coe,J
AU - Roy-Chowdhury,S
AU - Weierstall,U
AU - James,D
AU - Wang,D
AU - Grant,T
AU - Barty,A
AU - Yefanov,O
AU - Scales,J
AU - Gati,C
AU - Seuring,C
AU - Srajer,V
AU - Henning,R
AU - Schwander,P
AU - Fromme,R
AU - Ourmazd,A
AU - Moffat,K
AU - Van,Thor JJ
AU - Spence,JCH
AU - Fromme,P
AU - Chapman,HN
AU - Schmidt,M
DO - 10.1126/science.1259357
EP - 1246
PY - 2014///
SN - 1095-9203
SP - 1242
TI - Time-resolved serial crystallography captures high-resolution intermediates of photoactive yellow protein
T2 - Science
UR - http://dx.doi.org/10.1126/science.1259357
UR - http://www.sciencemag.org/content/346/6214/1242.abstract
VL - 346
ER -
