BibTex format
@article{Bryan:2014:10.1111/mmi.12826,
author = {Bryan, SJ and Burroughs, NJ and Shevela, D and Yu, J and Rupprecht, E and Liu, L-N and Mastroianni, G and Xue, Q and Llorente-Garcia, I and Leake, MC and Eichacker, LA and Schneider, D and Nixon, PJ and Mullineaux, CW},
doi = {10.1111/mmi.12826},
journal = {Molecular Microbiology},
pages = {1179--1195},
title = {Localisation and interactions of the Vipp1 protein in cyanobacteria},
url = {http://dx.doi.org/10.1111/mmi.12826},
volume = {94},
year = {2014}
}
RIS format (EndNote, RefMan)
TY - JOUR
AB - The Vipp1 protein is essential in cyanobacteria andchloroplasts for the maintenance of photosyntheticfunction and thylakoid membrane architecture. Toinvestigate its mode of action we generated strainsof the cyanobacteria Synechocystis sp. PCC6803and Synechococcus sp. PCC7942 in which Vipp1was tagged with green fluorescent protein at theC-terminus and expressed from the native chromosomallocus. There was little perturbation of function. Live-cell fluorescence imaging shows dramatic relocalisationof Vipp1 under high light. Under low light,Vipp1 is predominantly dispersed in the cytoplasmwith occasional concentrations at the outer peripheryof the thylakoid membranes. High light induces Vipp1coalescence into localised puncta within minutes, withnet relocation of Vipp1 to the vicinity of the cytoplasmicmembrane and the thylakoid membranes. Pulldownsand mass spectrometry identify an extensivecollection of proteins that are directly or indirectlyassociated with Vipp1 only after high-light exposure.These include not only photosynthetic and stressrelatedproteins but also RNA-processing, translationand protein assembly factors. This suggests that theVipp1 puncta could be involved in protein assembly.One possibility is that Vipp1 is involved in the formationof stress-induced localised protein assemblycentres, enabling enhanced protein synthesis anddelivery to membranes under stress conditions.
AU - Bryan,SJ
AU - Burroughs,NJ
AU - Shevela,D
AU - Yu,J
AU - Rupprecht,E
AU - Liu,L-N
AU - Mastroianni,G
AU - Xue,Q
AU - Llorente-Garcia,I
AU - Leake,MC
AU - Eichacker,LA
AU - Schneider,D
AU - Nixon,PJ
AU - Mullineaux,CW
DO - 10.1111/mmi.12826
EP - 1195
PY - 2014///
SN - 1365-2958
SP - 1179
TI - Localisation and interactions of the Vipp1 protein in cyanobacteria
T2 - Molecular Microbiology
UR - http://dx.doi.org/10.1111/mmi.12826
UR - http://hdl.handle.net/10044/1/28006
VL - 94
ER -
