BibTex format
@article{Horejs:2014:10.1073/pnas.1403139111,
author = {Horejs, C-M and Serio, A and Purvis, A and Gormley, AJ and Bertazzo, S and Poliniewicz, A and Wang, AJ and DiMaggio, P and Hohenester, E and Stevens, MM},
doi = {10.1073/pnas.1403139111},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
pages = {5908--5913},
title = {Biologically-active laminin-111 fragment that modulates the epithelial-to-mesenchymal transition in embryonic stem cells},
url = {http://dx.doi.org/10.1073/pnas.1403139111},
volume = {111},
year = {2014}
}
RIS format (EndNote, RefMan)
TY - JOUR
AB - The dynamic interplay between the extracellular matrix and embryonic stem cells (ESCs) constitutes one of the key steps in understanding stem cell differentiation in vitro. Here we report a biologically-active laminin-111 fragment generated by matrix metalloproteinase 2 (MMP2) processing, which is highly up-regulated during differentiation. We show that the β1-chain–derived fragment interacts via α3β1-integrins, thereby triggering the down-regulation of MMP2 in mouse and human ESCs. Additionally, the expression of MMP9 and E-cadherin is up-regulated in mouse ESCs—key players in the epithelial-to-mesenchymal transition. We also demonstrate that the fragment acts through the α3β1-integrin/extracellular matrix metalloproteinase inducer complex. This study reveals a previously unidentified role of laminin-111 in early stem cell differentiation that goes far beyond basement membrane assembly and a mechanism by which an MMP2-cleaved laminin fragment regulates the expression of E-cadherin, MMP2, and MMP9.
AU - Horejs,C-M
AU - Serio,A
AU - Purvis,A
AU - Gormley,AJ
AU - Bertazzo,S
AU - Poliniewicz,A
AU - Wang,AJ
AU - DiMaggio,P
AU - Hohenester,E
AU - Stevens,MM
DO - 10.1073/pnas.1403139111
EP - 5913
PY - 2014///
SN - 0027-8424
SP - 5908
TI - Biologically-active laminin-111 fragment that modulates the epithelial-to-mesenchymal transition in embryonic stem cells
T2 - Proceedings of the National Academy of Sciences of the United States of America
UR - http://dx.doi.org/10.1073/pnas.1403139111
UR - https://www.pnas.org/content/111/16/5908
UR - http://hdl.handle.net/10044/1/45486
VL - 111
ER -
