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Journal articleHo R, Hinder SJ, Watts JF, et al., 2010,
Determination of surface heterogeneity of D-mannitol by sessile drop contact angle and finite concentration inverse gas chromatography
, INTERNATIONAL JOURNAL OF PHARMACEUTICS, Vol: 387, Pages: 79-86, ISSN: 0378-5173- Author Web Link
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- Citations: 65
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Journal articleHare S, Gupta SS, Valkov E, et al., 2010,
Retroviral intasome assembly and inhibition of DNA strand transfer
, Nature, Vol: 464, Pages: 232-236, ISSN: 0028-0836Integrase is an essential retroviral enzyme that binds both termini of linear viral DNA and inserts them into a host cell chromosome. The structure of full-length retroviral integrase, either separately or in complex with DNA, has been lacking. Furthermore, although clinically useful inhibitors of HIV integrase have been developed, their mechanism of action remains speculative. Here we present a crystal structure of full-length integrase from the prototype foamy virus in complex with its cognate DNA. The structure shows the organization of the retroviral intasome comprising an integrase tetramer tightly associated with a pair of viral DNA ends. All three canonical integrase structural domains are involved in extensive protein–DNA and protein–protein interactions. The binding of strand-transfer inhibitors displaces the reactive viral DNA end from the active site, disarming the viral nucleoprotein complex. Our findings define the structural basis of retroviral DNA integration, and will allow modelling of the HIV-1 intasome to aid in the development of antiretroviral drugs.
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Journal articleHelaine S, Thompson JA, Watson KG, et al., 2010,
Dynamics of intracellular bacterial replication at the single cell level
, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 107, Pages: 3746-3751, ISSN: 0027-8424- Author Web Link
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- Citations: 222
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Journal articleTelfer A, Pascal AA, Bordes L, et al., 2010,
Fluorescence Line Narrowing Studies on Isolated Chlorophyll Molecules
, JOURNAL OF PHYSICAL CHEMISTRY B, Vol: 114, Pages: 2255-2260, ISSN: 1520-6106- Author Web Link
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- Citations: 14
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Journal articleCamara B, Liu M, Reynolds J, et al., 2010,
T7 phage protein Gp2 inhibits the <i>Escherichia coli</i> RNA polymerase by antagonizing stable DNA strand separation near the transcription start site
, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 107, Pages: 2247-2252, ISSN: 0027-8424- Author Web Link
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- Citations: 53
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Journal articleEwens CA, Kloppsteck P, Foerster A, et al., 2010,
Structural and functional implications of phosphorylation and acetylation in the regulation of the AAA+ protein p97
, BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE, Vol: 88, Pages: 41-48, ISSN: 0829-8211- Author Web Link
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- Citations: 30
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Journal articleChaudhuri RR, Sebaihia M, Hobman JL, et al., 2010,
Complete Genome Sequence and Comparative Metabolic Profiling of the Prototypical Enteroaggregative <i>Escherichia coli</i> Strain 042
, PLOS ONE, Vol: 5, ISSN: 1932-6203- Author Web Link
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- Citations: 133
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Journal articleZunszain PA, Knox SR, Sweeney TR, et al., 2010,
Insights into Cleavage Specificity from the Crystal Structure of Foot-and-Mouth Disease Virus 3C Protease Complexed with a Peptide Substrate
, JOURNAL OF MOLECULAR BIOLOGY, Vol: 395, Pages: 375-389, ISSN: 0022-2836- Author Web Link
- Open Access Link
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- Citations: 53
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Journal articleKhoo JY, Heng JYY, Williams DR, 2010,
Agglomeration Effects on the Drying and Dehydration Stability of Pharmaceutical Acicular Hydrate: Carbamazepine Dihydrate
, INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH, Vol: 49, Pages: 422-427, ISSN: 0888-5885- Author Web Link
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- Citations: 12
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Journal articleHughes S, Jenkins V, Dar MJ, et al., 2010,
Transcriptional Co-activator LEDGF Interacts with Cdc7-Activator of S-phase Kinase (ASK) and Stimulates Its Enzymatic Activity
, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 285, Pages: 541-554- Author Web Link
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- Citations: 50
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Journal articleCurry S, 2010,
More light and colour. Science and the new media
, Biochemist, Vol: 32, Pages: 18-21, ISSN: 0954-982X -
Journal articleThompson O, Moore CJ, Hussaina S-A, et al., 2010,
Modulation of cell spreading and cell-substrate adhesion dynamics by dystroglycan
, JOURNAL OF CELL SCIENCE, Vol: 123, Pages: 118-127, ISSN: 0021-9533- Author Web Link
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- Citations: 42
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Conference paperRobinson T, Manning HB, Dunsby C, et al., 2010,
Investigating fast enzyme-DNA kinetics using multidimensional fluorescence imaging and microfluidics
, Conference on Microfluidics, BioMEMS, and Medical Microsystems VIII, Publisher: SPIE-INT SOC OPTICAL ENGINEERING, ISSN: 0277-786X- Author Web Link
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- Citations: 1
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Journal articleVittadello M, Gorbunov MY, Mastrogiovanni DT, et al., 2010,
Photoelectron Generation by Photosystem II Core Complexes Tethered to Gold Surfaces
, CHEMSUSCHEM, Vol: 3, Pages: 471-475, ISSN: 1864-5631- Author Web Link
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- Citations: 29
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Journal articleThomas JC, Green JL, Howson RI, et al., 2010,
Interaction and dynamics of the <i>Plasmodium</i> <i>falciparum</i> MTIP-MyoA complex, a key component of the invasion motor in the malaria parasite
, MOLECULAR BIOSYSTEMS, Vol: 6, Pages: 494-498, ISSN: 1742-206X- Author Web Link
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- Citations: 25
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Journal articleAyub M, Ivanov A, Instuli E, et al., 2010,
Nanopore/electrode structures for single-molecule biosensing
, Electrochimica Acta, Vol: 55, Pages: 8237-8243 -
Journal articleHerrero C, Hughes JL, Quaranta A, et al., 2010,
Intramolecular light induced activation of a Salen-Mn<SUP>III</SUP> complex by a ruthenium photosensitizer
, CHEMICAL COMMUNICATIONS, Vol: 46, Pages: 7605-7607, ISSN: 1359-7345- Author Web Link
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- Citations: 30
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Journal articleRoskilly SJ, Colbourn EA, Alli O, et al., 2010,
Investigating the effect of shape on particle segregation using aMonte Carlo simulation
, Powder Technology, Vol: 203, Pages: 211-222A Monte Carlo simulation has been used to investigate the segregation potential of a range of particulate systems under conditions in which the particles undergo high amplitude low frequency shaking. These systems involve a wide range of binary powder mixtures in which complex particle shapes have been investigated, including plates and rods which represent the real world materials encountered in pharmaceutical systems such as those which include crystalline components. Previous simulations on the segregation propensity of systems with different shapes were limited to spheres and spherocylinders, with relatively low vibrational amplitude drops. A commercial computer application for particle packing—calledMacroPac—has been successfully employed here, as it has been able to model systems that are more complex where the shape variation is much wider. These simulations apply to the case of macroscopic particles, in the absence of air resistance and inter-particle forces. For non-spherical shapes, an ‘effective size’ which relates to the radius of gyration of the particles is determined. Our studies indicate that with high amplitude low frequency shaking, in a mixture of particles with different shapes but with equal volumes, the particles with the larger ‘effective size’, which tend to have a lower packing fraction, segregate to the top
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Journal articleLow HH, Sachse C, Amos LA, et al., 2009,
Structure of a Bacterial Dynamin-like Protein Lipid Tube Provides a Mechanism For Assembly and Membrane Curving
, CELL, Vol: 139, Pages: 1342-1352, ISSN: 0092-8674- Author Web Link
- Open Access Link
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- Citations: 136
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Journal articleDouzi B, Durand E, Bernard C, et al., 2009,
The XcpV/GspI Pseudopilin Has a Central Role in the Assembly of a Quaternary Complex within the T2SS Pseudopilus
, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 284, Pages: 34580-34589- Author Web Link
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- Citations: 51
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Journal articleCarafoli F, Bihan D, Stathopoulos S, et al., 2009,
Crystallographic Insight into Collagen Recognition by Discoidin Domain Receptor 2
, STRUCTURE, Vol: 17, Pages: 1573-1581, ISSN: 0969-2126- Author Web Link
- Open Access Link
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- Citations: 105
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Journal articleEswaran J, Patnaik D, Filippakopoulos P, et al., 2009,
Structure and functional characterization of the atypical human kinase haspin
, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 106, Pages: 20198-20203, ISSN: 0027-8424- Author Web Link
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- Citations: 122
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Journal articleNicastro G, Masino L, Esposito V, et al., 2009,
Josephin Domain of Ataxin-3 Contains Two Distinct Ubiquitin-Binding Sites
, BIOPOLYMERS, Vol: 91, Pages: 1203-1214, ISSN: 0006-3525- Author Web Link
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- Citations: 71
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Journal articleListorti A, Durrant J, Barber J, 2009,
ARTIFICIAL PHOTOSYNTHESIS Solar to fuel
, NATURE MATERIALS, Vol: 8, Pages: 929-U22, ISSN: 1476-1122- Author Web Link
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- Citations: 189
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Journal articleBulgin R, Arbeloa A, Goulding D, et al., 2009,
The T3SS Effector EspT Defines a New Category of Invasive Enteropathogenic <i>E</i>. <i>coli</i> (EPEC) Which Form Intracellular Actin Pedestals
, PLOS PATHOGENS, Vol: 5, ISSN: 1553-7366- Author Web Link
- Open Access Link
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- Citations: 42
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Journal articleDe Simone A, Cavalli A, Hsu S-TD, et al., 2009,
Accurate Random Coil Chemical Shifts from an Analysis of Loop Regions in Native States of Proteins
, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, Vol: 131, Pages: 16332-+, ISSN: 0002-7863- Author Web Link
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- Citations: 78
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Journal articleBaier J, Gabrielsen M, Oellerich S, et al., 2009,
Spectral Diffusion and Electron-Phonon Coupling of the B800 BChl a Molecules in LH2 Complexes from Three Different Species of Purple Bacteria
, BIOPHYSICAL JOURNAL, Vol: 97, Pages: 2604-2612, ISSN: 0006-3495- Author Web Link
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- Citations: 22
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Journal articleChim N, McMath LM, Beeby M, et al., 2009,
Advances in Mycobacterium tuberculosis structural genomics: investigating potential chinks in the armor of a deadly pathogen
, Infect Disord Drug Targets, Vol: 9, Pages: 475-492 -
Journal articleBolanos-Garcia VM, Chayen NE, 2009,
New directions in conventional methods of protein crystallization
, PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY, Vol: 101, Pages: 3-12, ISSN: 0079-6107- Author Web Link
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- Citations: 44
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Journal articleHu Y, Guimond SE, Travers P, et al., 2009,
Novel Mechanisms of Fibroblast Growth Factor Receptor 1 Regulation by Extracellular Matrix Protein Anosmin-1
, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 284, Pages: 29905-29920- Author Web Link
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- Citations: 59
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