Publications

Search or filter publications

Search publications Search

Filter by type:

Filter by publication type

• Book
• Book chapter
• Conference paper
• Journal article
• Patent
• Report
• Software

Filter by year:

Filter from 20212020201920182017201620152014201320122011201020092008 to Filter to 20212020201920182017201620152014201320122011201020092008

---

Search results

• Journal article
  Maertens GN, Hare S, Cherepanov P, 2010,

  The mechanism of retroviral integration from X-ray structures of its key intermediates

  , Nature, Vol: 468, Pages: 326-U217, ISSN: 0028-0836

  To establish productive infection, a retrovirus must insert a DNA replica of its genome into host cell chromosomal DNA1,2. This process is operated by the intasome, a nucleoprotein complex composed of an integrase tetramer (IN) assembled on the viral DNA ends3,4. The intasome engages chromosomal DNA within a target capture complex to carry out strand transfer, irreversibly joining the viral and cellular DNA molecules. Although several intasome/transpososome structures from the DDE(D) recombinase superfamily have been reported4,5,6, the mechanics of target DNA capture and strand transfer by these enzymes remained unclear. Here we report crystal structures of the intasome from prototype foamy virus in complex with target DNA, elucidating the pre-integration target DNA capture and post-catalytic strand transfer intermediates of the retroviral integration process. The cleft between IN dimers within the intasome accommodates chromosomal DNA in a severely bent conformation, allowing widely spaced IN active sites to access the scissile phosphodiester bonds. Our results resolve the structural basis for retroviral DNA integration and provide a framework for the design of INs with altered target sequences.

  • Abstract
  • Publisher Web Link
  • Author Web Link
  • Open Access Link
  • Cite
• Journal article
  Schroeder GN, Petty NK, Mousnier A, Harding CR, Vogrin AJ, Wee B, Fry NK, Harrison TG, Newton HJ, Thomson NR, Beatson SA, Dougan G, Hartland EL, Frankel Get al., 2010,

  <i>Legionella pneumophila</i> Strain 130b Possesses a Unique Combination of Type IV Secretion Systems and Novel Dot/Icm Secretion System Effector Proteins

  , JOURNAL OF BACTERIOLOGY, Vol: 192, Pages: 6001-6016, ISSN: 0021-9193
  • Author Web Link
  • Cite
  • Citations: 90
• Journal article
  Choudhury HG, Beis K, 2010,

  Crystallization and initial X-ray diffraction analysis of the tellurite-resistance <i>S</i>-adenosyl-l-methionine transferase protein TehB from <i>Escherichia coli</i>

  , ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, Vol: 66, Pages: 1496-1499
  • Author Web Link
  • Cite
  • Citations: 4
• Journal article
  Ramboarina S, Garnett JA, Zhou M, Li Y, Peng Z, Taylor JD, Lee W-C, Bodey A, Murray JW, Alguel Y, Bergeron J, Bardiaux B, Sawyer E, Isaacson R, Tagliaferri C, Cota E, Nilges M, Simpson P, Ruiz T, Wu H, Matthews Set al., 2010,

  Structural Insights into Serine-rich Fimbriae from Gram-positive Bacteria

  , JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 285, Pages: 32446-32457, ISSN: 0021-9258
  • Author Web Link
  • Open Access Link
  • Cite
  • Citations: 42
• Journal article
  Ryan AJ, Ghuman J, Zunszain PA, Chung C-W, Curry Set al., 2010,

  Structural basis of binding of fluorescent, site-specific dansylated amino acids to human serum albumin

  , Journal of Structural Biology, Vol: 174, Pages: 84-91, ISSN: 1095-8657
  • Open Access Link
  • Cite
• Journal article
  Vossenkaemper A, Marches O, Fairclough PD, Warnes G, Stagg AJ, Lindsay JO, Evans PC, Luong LA, Croft NM, Naik S, Frankel G, MacDonald TTet al., 2010,

  Inhibition of NF-κB Signaling in Human Dendritic Cells by the Enteropathogenic <i>Escherichia coli</i> Effector Protein NleE

  , JOURNAL OF IMMUNOLOGY, Vol: 185, Pages: 4118-4127, ISSN: 0022-1767
  • Author Web Link
  • Cite
  • Citations: 67
• Journal article
  Karatsa-Dodgson M, Woermann ME, Gruendling A, 2010,

  <i>In Vitro</i> Analysis of the <i>Staphylococcus aureus</i> Lipoteichoic Acid Synthase Enzyme Using Fluorescently Labeled Lipids

  , JOURNAL OF BACTERIOLOGY, Vol: 192, Pages: 5341-5349, ISSN: 0021-9193
  • Author Web Link
  • Cite
  • Citations: 30
• Journal article
  Watson KG, Holden DW, 2010,

  Dynamics of growth and dissemination of <i>Salmonella in vivo</i>

  , CELLULAR MICROBIOLOGY, Vol: 12, Pages: 1389-1397, ISSN: 1462-5814
  • Author Web Link
  • Cite
  • Citations: 97
• Journal article
  Yan R, Simpson PJ, Matthews SJ, Cota Eet al., 2010,

  Backbone <SUP>1</SUP>H, <SUP>15</SUP>N, <SUP>13</SUP>C and Ile, Leu, Val methyl chemical shift assignments for the 33.5 kDa N-terminal domain of Candida albicans ALS1

  , BIOMOLECULAR NMR ASSIGNMENTS, Vol: 4, Pages: 187-190, ISSN: 1874-2718
  • Author Web Link
  • Cite
  • Citations: 8
• Journal article
  Michoux F, Takasaka K, Boehm M, Nixon PJ, Murray JWet al., 2010,

  Structure of CyanoP at 2.8 Å: Implications for the Evolution and Function of the PsbP Subunit of Photosystem II

  , BIOCHEMISTRY, Vol: 49, Pages: 7411-7413, ISSN: 0006-2960
  • Author Web Link
  • Cite
  • Citations: 43
• Journal article
  Krishnan L, Li X, Naraharisetty HL, Hare S, Cherepanov P, Engelman Aet al., 2010,

  Structure-based modeling of the functional HIV-1 intasome and its inhibition

  , PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 107, Pages: 15910-15915, ISSN: 0027-8424
  • Author Web Link
  • Cite
  • Citations: 171
• Journal article
  Bush M, Ghosh T, Tucker N, Zhang X, Dixon Ret al., 2010,

  Nitric oxide-responsive interdomain regulation targets the σ54-interaction surface in the enhancer binding protein NorR

  , MOLECULAR MICROBIOLOGY, Vol: 77, Pages: 1278-1288, ISSN: 0950-382X
  • Author Web Link
  • Cite
  • Citations: 18
• Journal article
  Berger CN, Sodha SV, Shaw RK, Griffin PM, Pink D, Hand P, Frankel Get al., 2010,

  Fresh fruit and vegetables as vehicles for the transmission of human pathogens

  , ENVIRONMENTAL MICROBIOLOGY, Vol: 12, Pages: 2385-2397, ISSN: 1462-2912
  • Author Web Link
  • Cite
  • Citations: 594
• Journal article
  McClelland A, Demidov A, Benabbas A, Sun Y, Venugopal K, Sage T, van Thor J, Champion Pet al., 2010,

  Investigation of excited state proton transfer in green fluorescent protein

  , ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, Vol: 240, ISSN: 0065-7727
  • Author Web Link
  • Cite
• Journal article
  Sweeney TR, Cisnetto V, Bose D, Bailey M, Wilson JR, Zhang X, Belsham GJ, Curry Set al., 2010,

  Foot-and-Mouth Disease Virus 2C Is a Hexameric AAA plus Protein with a Coordinated ATP Hydrolysis Mechanism

  , JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 285, Pages: 24347-24359
  • Author Web Link
  • Open Access Link
  • Cite
  • Citations: 50
• Journal article
  Dumont A, Boucrot E, Drevensek S, Daire V, Gorvel J-P, Poues C, Holden DW, Meresse Set al., 2010,

  SKIP, the Host Target of the <i>Salmonella</i> Virulence Factor SifA, Promotes Kinesin-1-Dependent Vacuolar Membrane Exchanges

  , TRAFFIC, Vol: 11, Pages: 899-911, ISSN: 1398-9219
  • Author Web Link
  • Cite
  • Citations: 82
• Journal article
  Munera D, Crepin VF, Marches O, Frankel Get al., 2010,

  N-Terminal Type III Secretion Signal of Enteropathogenic <i>Escherichia coli</i> Translocator Proteins

  , JOURNAL OF BACTERIOLOGY, Vol: 192, Pages: 3534-3539, ISSN: 0021-9193
  • Author Web Link
  • Cite
  • Citations: 29
• Journal article
  Sadovskaya I, Vinogradov E, Li J, Hachani A, Kowalska K, Filloux Aet al., 2010,

  High-level antibiotic resistance in <i>Pseudomonas aeruginosa</i> biofilm: the <i>ndvB</i> gene is involved in the production of highly glycerol-phosphorylated β-(1→3)-glucans, which bind aminoglycosides

  , GLYCOBIOLOGY, Vol: 20, Pages: 895-904, ISSN: 0959-6658
  • Author Web Link
  • Cite
  • Citations: 78
• Journal article
  Nixon PJ, Michoux F, Yu J, Boehm M, Komenda Jet al., 2010,

  Recent advances in understanding the assembly and repair of photosystem II

  , ANNALS OF BOTANY, Vol: 106, Pages: 1-16, ISSN: 0305-7364
  • Author Web Link
  • Cite
  • Citations: 405
• Journal article
  Salacha R, Kovacic F, Brochier-Armanet C, Wilhelm S, Tommassen J, Filloux A, Voulhoux R, Bleves Set al., 2010,

  The <i>Pseudomonas aeruginosa</i> patatin-like protein PlpD is the archetype of a novel Type V secretion system

  , ENVIRONMENTAL MICROBIOLOGY, Vol: 12, Pages: 1498-1512, ISSN: 1462-2912
  • Author Web Link
  • Cite
  • Citations: 73
• Journal article
  Kertis F, Snyder J, Govada L, Khurshid S, Chayen N, Erlebacher Jet al., 2010,

  Structure/Processing Relationships in the Fabrication of Nanoporous Gold

  , JOM, Vol: 62, Pages: 50-56, ISSN: 1047-4838
  • Author Web Link
  • Cite
  • Citations: 79
• Journal article
  Robinson KS, Mousnier A, Hemrajani C, Fairweather N, Berger CN, Frankel Get al., 2010,

  The enteropathogenic <i>Escherichia coli</i> effector NleH inhibits apoptosis induced by <i>Clostridium difficile</i> toxin B

  , MICROBIOLOGY-SGM, Vol: 156, Pages: 1815-1823, ISSN: 1350-0872
  • Author Web Link
  • Cite
  • Citations: 23
• Journal article
  Yu X-J, McGourty K, Liu M, Unsworth KE, Holden DWet al., 2010,

  pH Sensing by Intracellular <i>Salmonella</i> Induces Effector Translocation

  , SCIENCE, Vol: 328, Pages: 1040-1043, ISSN: 0036-8075
  • Author Web Link
  • Open Access Link
  • Cite
  • Citations: 137
• Journal article
  Chen S, McDowall A, Dobro MJ, Briegel A, Ladinsky M, Shi J, Tocheva EI, Beeby M, Pilhofer M, Ding HJ, Li Z, Gan L, Morris DM, Jensen GJet al., 2010,

  Electron cryotomography of bacterial cells

  , Jove-Journal of Visualized Experiments, Vol: 39, ISSN: 1940-087X

  While much is already known about the basic metabolism of bacterial cells, many fundamental questions are still surprisingly unanswered,including for instance how they generate and maintain specific cell shapes, establish polarity, segregate their genomes, and divide. In orderto understand these phenomena, imaging technologies are needed that bridge the resolution gap between fluorescence light microscopy andhigher-resolution methods such as X-ray crystallography and NMR spectroscopy.Electron cryotomography (ECT) is an emerging technology that does just this, allowing the ultrastructure of cells to be visualized in a near-nativestate, in three dimensions (3D), with "macromolecular" resolution (~4nm).1, 2 In ECT, cells are imaged in a vitreous, "frozen-hydrated" state ina cryo transmission electron microscope (cryoTEM) at low temperature (< -180°C). For slender cells (up to ~500 nm in thickness3), intact cellsare plunge-frozen within media across EM grids in cryogens such as ethane or ethane/propane mixtures. Thicker cells and biofilms can alsobe imaged in a vitreous state by first "high-pressure freezing" and then, "cryo-sectioning" them. A series of two-dimensional projection imagesare then collected through the sample as it is incrementally tilted along one or two axes. A three-dimensional reconstruction, or "tomogram" canthen be calculated from the images. While ECT requires expensive instrumentation, in recent years, it has been used in a few labs to reveal thestructures of various external appendages, the structures of different cell envelopes, the positions and structures of cytoskeletal filaments, andthe locations and architectures of large macromolecular assemblies such as flagellar motors, internal compartments and chemoreceptor arrays.1,2In this video article we illustrate how to image cells with ECT, including the processes of sample preparation, data collection, tomogramreconstruction, and interpre

  • Abstract
  • Open Access Link
  • Cite
• Journal article
  Klopfleisch C, Minh LQ, Giesow K, Curry S, Keil GMet al., 2010,

  Effect of foot-and-mouth disease virus capsid precursor protein and 3C protease expression on bovine herpesvirus 1 replication

  , ARCHIVES OF VIROLOGY, Vol: 155, Pages: 723-731, ISSN: 0304-8608
  • Author Web Link
  • Cite
  • Citations: 4
• Journal article
  Cherepanov P, 2010,

  Integrase illuminated

  , EMBO REPORTS, Vol: 11, Pages: 328-328, ISSN: 1469-221X
  • Author Web Link
  • Cite
  • Citations: 20
• Journal article
  Komenda J, Knoppova J, Krynicka V, Nixon PJ, Tichy Met al., 2010,

  Role of FtsH2 in the repair of Photosystem II in mutants of the cyanobacterium <i>Synechocystis</i> PCC 6803 with impaired assembly or stability of the CaMn₄ cluster

  , BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, Vol: 1797, Pages: 566-575, ISSN: 0005-2728
  • Author Web Link
  • Cite
  • Citations: 37
• Journal article
  Sciara G, Bebeacua C, Bron P, Tremblay D, Ortiz-Lombardia M, Lichiere J, van Heel M, Campanacci V, Moineau S, Cambillau Cet al., 2010,

  Structure of lactococcal phage p2 baseplate and its mechanism of activation

  , PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 107, Pages: 6852-6857, ISSN: 0027-8424
  • Author Web Link
  • Cite
  • Citations: 122
• Journal article
  Rasool N, Yasin T, Heng JYY, Akhter Zet al., 2010,

  Synthesis and characterization of novel pH-, ionic strength and temperature- sensitive hydrogel for insulin delivery

  , POLYMER, Vol: 51, Pages: 1687-1693, ISSN: 0032-3861
  • Author Web Link
  • Cite
  • Citations: 124
• Journal article
  Beale J, Lee SY, Iwata S, Beis Ket al., 2010,

  Structure of the aliphatic sulfonate-binding protein SsuA from <i>Escherichia coli</i>

  , ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, Vol: 66, Pages: 391-396
  • Author Web Link
  • Cite
  • Citations: 8

This data is extracted from the Web of Science and reproduced under a licence from Thomson Reuters. You may not copy or re-distribute this data in whole or in part without the written consent of the Science business of Thomson Reuters.